Abstract

"A simple and cost-effective bioremediation strategy for the clean-up of a polluted Egyptian sandy soil was developed . Non-sterile bagasse with NP fertilizer and soybean meal without NP fertilizer were promising and cost-effective treatments for the development of higher counts of THB and HDM, which led to enhanced biodegradation of the spent motor oil (60.1±3.1% and 58.6 ± 2.3% respectively). Six bacterial isolates showed good growth in the presence of spent motor oil. They were identified as: Arthrobacter sp (EM2), Bacillus subtilis (EM6) , Bacillus sp (EM10), Corynebacterium sp (EM 14), Pseudomonas aeruginosa (EM1) and Pseudomonas sp (EM19). Pseudomonas aeruginosa (EM1) showed higher biodegradation activity (68.0 ± 2.0%), this was followed by Bacillus subtilis (60.3 ± 3.4%). The mixed culture of the six bacterial strains increased the biodegradation process to 76.3 ± 3.2 % (w/w), this may represents a successful approach for the bioremediation of spent-motor oil polluted sites . "

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