Application of Pup1 Markers for Identification of Rice (Oryza Sativa L.) Cultivars with Tolerance to Phosphorus Deficiency
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 4
Abstract
Phosphorus deficiency (PD) often exists in soils that severely limit rice growth and production due to fixation in soils with high free ferric oxides and aluminum in the clay fraction. It is a widespread problem and limits access of plants to Phosphorus even if it is present in the soil. P-efficient varieties should play a major role in increasing rice yield. Therefore in this study, a set of 120 rice genotypes (composed of upland cultivars and landraces) were grown in low P soils with three different P fertilizer dose inputs (P0, P25 and P50 kgha-1 P) to compare P uptake efficiency. Results showed that, among the 120 genotypes, fifteen genotypes (12.5%) which produced high grain yield were further screened for probable presence of Pup1 gene by PCR amplification by two Pup1 associated insertion and deletion (InDel) markers viz, Pup1-K46 and Pup1-K52. Among the fifteen genotypes PCR screening confirmed the presence of Pup1 gene in more than 90% of the genotypes. In summary, the identified genotypes may serve as sources of PD tolerance that would be useful in future rice breeding programme.
Authors and Affiliations
S. Vellaikumar, P. Malarvizhi
Keywords
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