ASSAY OF FOUR PSYCHOTROPIC DRUGS CHLORPROMAZINE, CLOMIPRAMINE, AMITRIPTYLINE AND NORTRIPTYLINE IN TABLETS BY A SINGLE HPLC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: The objective of the study was to develop and validate a novel and single HPLC method, which is a additional practical choice in quality control laboratories, in order to assay four psychotropic drugs chlorpromazine, clomipramine, amitriptyline and nortriptyline in tablets.Methods: The chromatographic conditions comprised of a classical C8-type stationary phase (250 × 4.6 mm, 5μ), with a mobile phase consisting of 3g of sodium lauryl sulfate in a mixture of 400 ml of deionized water and 600 ml of acetonitrile, then 0.5 g of ammonium nitrate was added and apparent pH of 3.0 was adjusted with glacial acetic acid. The flow rate was 1 ml/min; the detection wavelength was at 254 nm under a temperature of 22 ○C.Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products and excipients. Then it was used successfully to the assay of these drugs in tablets purchased from Syria. These analyzed tablets contained the active ingredient within 100±5% of stated concentration, within the limits specified by British Pharmacopeia.Conclusion: The results presented in this paper showed that the developed method was simple and practical for the determination of the four drugs in tablets.
Authors and Affiliations
Saleh Trefi
Keywords
Related Articles
- EP ID EP576128
- DOI -
- Views 62
- Downloads 0
SYNTHESIS AND ANTICANCER SCREENING OF TRIAZINE ANALOGUES
Objective: The study was aimed to investigate the cytotoxic effect of S-5H-[1,2,4]-triazino (5,6-b) indol-3-yl-3,4-phenylethane-thioate derivatives as epidermal growth factor Receptor (EGFR) inhibitors. Methods: In the...
ANTIOXIDANT STUDIES ON METHANOL AND AQUEOUS EXTRACTS OF GYMNOSPORIA MONTANA PLANT
Objective: The main objective of this study was to evaluate the free radical scavenging activity of methanol (70%) and aqueous extract of G. montana leaves which is a traditionally used herb known for its hepatoprotectiv...
DOCKING AND CYTOTOXICITY STUDIES OF 2-VINYLCHROMONE DERIVATIVES ON HUMAN BREAST CANCER CELL LINES
Objective: Estrogen receptor (ER) is over-expressed in 70% of breast cancers. The ER has two isoforms, ERα and ERβ. The ER ligand binding domain (LBD) has been the target for hormone-responsive breast cancer. Due to ti...
GC MS AND ELEMENTAL ANALYSIS OF CINNAMOMUM TAMALA
Objective: To investigate the phytoconstituents and elements present in hexane (HEX), dichloromethane (DCM) and methanol (MET) extracts of Cinnamomum tamala (CT).Methods: Gas chromatography–mass spectrometry (GC-MS) wa...
PHARMACOGNOSTICAL AND PHYTOCHEMICAL STANDARDIZATION OF PHYSALIS MINIMA L. LEAF
Objective: To evaluate the pharmacognostical and phytochemical parameters of Physalis minima leaf. Methods: The leaf of Physalis minima was examined for macroscopical, microscopical, physicochemical parameters and fluor...