Assessing the Performance Characteristics of the “CareStartTM Malaria HRP2 pf (CAT NO: G0141, ACCESSBIO)” Rapid Diagnostic Test for Asymptomatic Malaria in Mutengene, Cameroon
Journal Title: International Journal of TROPICAL DISEASE & Health - Year 2014, Vol 4, Issue 9
Abstract
Aim: The aim of this study was to determine the prevalence and density of malaria parasites in asymptomatic school children in Mutengene and evaluate the performance characteristics of the ‘CareStartTM Malaria HRP2 pf (CAT NO: G0141, ACCESSBIO)’ rapid diagnostic test (RDT) using light microscopy as a gold standard. Study Design: The study was a cross-sectional survey. Place and Duration of Study: The study was carried out in Mutengene, from February to March, 2013. Methodology: A total of 406 pupils were studied. Demographic data was taken for each child and capillary blood was collected. Blood films were prepared for the assessment of parasite density and speciation. A drop of blood was used on the RDT to determine the malaria status. Results: The mean age at 95% confidence interval (CI) was 8 ± 2 years (range = 4 -15 years) and the overall prevalence of malaria was 39.9% (162) by microscopy. The geometric mean parasite density (GMPD) was 2332.7 parasites/µL (range: 218 - 16000). Only 386 pupils were examined by both methods. More pupils were positive by microscopy (40.9%, CI = 36.1 - 45.9) than by RDT (27.9%, CI = 23.7 - 32.7) and the difference was statistically significant (χ2 = 16.1, P <0.0001). The majority of those detected had high infection (≥ 5000 parasite/µL). Less than 50% of those with low (25.0%, CI = 12.0 - 44.9), moderate (40.7%, CI = 32.24-49.70) and high parasitaemia (75%, CI = 5.00-89.82) were positive by RDT and the difference was significant (χ2 = 10.09, P = 0.006). The RDT showed a low sensitivity of 48.5% (CI = 40.3 – 56.9%) and specificity of 84.0% (CI = 80.0- 88.2%). Conclusion: More research needs to be done on the RDT to improve on its performance characteristics before it could be used in mass surveillance programmes.
Authors and Affiliations
Judith Lum Ndamukong-Nyanga, Helen K. Kimbi, Irene Ule Ngole Sumbele, Lum Emmaculate, Malaika Nain Nweboh, Yannick Nana, Sunjo Cyrilla Bertek, Kenneth J. N. Ndamukong
Keywords
Related Articles
- EP ID EP343444
- DOI 10.9734/IJTDH/2014/10778
- Views 84
- Downloads 0
Clinical and Laboratory Profile of ARV Naive HIV Infected Children in the Era of Highly Active Anti-retroviral Therapy in Enugu, South-East Nigeria
Background: HIV/AIDS is one of the most dynamic epidemic infectious diseases. An estimated 1000 children are newly infected with HIV every day, most of them in sub-Saharan Africa. They often present with various clinical...
Prevalence of Dental Fluorosis among Secondary School Children in Oloibiri Community
Background: Dental fluorosis with its attendant complication of aesthetic distortion of the teeth has now become another focus of public health intervention worldwide. We studied the prevalence of dental fluorosis among...
Lesion Aspirate Isolation, Culture, Diagnosis and Molecular Identification of Cutaneous Leishmaniasis in District Kohat, Khyber Pakhtunkhwa, Pakistan
Leishmaniasis is a poverty-associated zoonotic tropical disease transmitted by the bite of infected female sand fly. Diagnosis and recognition of clinical manifestation and morphological features of Leishmania parasites...
Prevalence of Intestinal Protozoan Infections and the Associated Risk Factors among Children in Bushenyi District, Western Uganda
Aims: To determine the prevalence of intestinal protozoan infections and the associated risk factors in children. Study Design: A cross sectional study. Place and Duration of Study: The study was conducted in four select...
Use of Insecticide-treated Net and Malaria Parasitemia in Under-fives at National Hospital Abuja
Aim: To determine the relationship of ITN utilisation with malaria parasitemia in under-five children attending the General Paediatric Outpatient Department (GPOPD) in National Hospital Abuja (NHA), as well as, explore f...