ASSESSING THE THERAPEUTIC ROLE OF JOSHANDA: PHYTOCHEMICAL, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITIES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 2
Abstract
Objective: Joshanda, a polyherbal Unani formulation is extensively used as a common home remedy for the treatment of a cough and cold accompanied by pharyngeal inflammation and fever. This study aimed to analyze phytochemicals, antioxidant, anti-inflammatory, and antibacterial activity.Methods: The study investigated the presence of phyto-compounds in joshanda and antioxidant, antibacterial, anti-fungal, and anti-inflammatory activities by various in vitro standard methods using ascorbic acid, ampicillin, and aspirin respectively as standard drugs.Results: Joshanda aqueous extract revealed the presence of tannins, phenols, flavonoids glycosides, terpenoids, and alkaloids and absence of sterols, saponins, xanthoprotiens, and carboxylic acid. Joshanda showed the highest inhibition against B. subtilis (% MGI 99.000±0.577) and least inhibition against P. aeruginosa (%MGI of 84.102±0.491). Joshanda extract, ascorbic acid demonstrated highest % DPPH radical scavenging of 98.379±0.313%, 98.843±0.443% and a minimum of 36.210±1.174%, 83.192±0.422%. Results showed H2O2 scavenging activity of 0.047±0.001 μg/ml per minute degradation of H2O2. FRAP value was observed in joshanda and ascorbic acid with a maximum of 0.945±0.024, 0.687±0.047 mmol and minimum of 0.171±0.036, 0.059±0.005 mmol respectively. Joshanda extract showed the highest albumin denaturation inhibition of 14.069±0.350% and the lowest of 1.880±0.194% at extract volume of 1000 µl and 100 µl respectively. The extract demonstrated the highest proteinase inhibition of 24.003±0.291 % and the lowest of 4.959±0.254% comparable to aspirin. Joshanda had no potent anticandidal activity up to 1 mg/ml.Conclusion: Results clearly suggested that joshanda is a potent phytodrug and can also be used as a strong reactive oxygen species scavenger, might be used as anti-arthritic and strong natural antibiotic agent for effective treatment of various oxidative stressed disorders.
Authors and Affiliations
Tooba Naz Shamsi, Romana Parveen, Archoo Sajida, Afaque Ahmad, Sadaf Fatima
Keywords
Related Articles
- EP ID EP575377
- DOI 10.22159/ijpps.2018v10i2.22450
- Views 77
- Downloads 0
THE EFFECT OF ROSELLA (HIBISCUS SABDARIFFA L) TREATMENT ON IL-10 AND IL-14 SECRETION ON DIMETHYLBENZ (A) ANTHRACENE (DMBA) INDUCED RAT
Objective: The objective of this study was to determine the effect of ethanolic extract rosella calyx pretreatment on increasing secretion of IL-10 and IL-14 on SD rats treated with DMBA.Methods: Rats were treated with a...
DETECTION OF HPV TYPE 45 L2 GENE IN CERVICAL CANCER PATIENTS BY POLYMERASE CHAIN REACTION METHOD
Objective: Cervical cancer is the second common type of cancer among women in the Indonesian population. Persistent infection with high risk human papillomavirus (hrHPV) has been considered as the most significant risk f...
ISOLATION AND SCREENING OF MARINE MICROALGAE CHLORELLA SP. _PR1 FOR ANTICANCER ACTIVITY
Objective: The objective of the present study includes isolation, characterization and screening of anticancerous activity against B16F10 cell line using isolated marine microalgae Chlorella sp._PR1.Methods: In this stud...
CYTOTOXIC SCREENING OF THREE EGYPTIAN PLANTS USING BRINE SHRIMP LETHALITY TEST
Objective: Brine shrimp (Artemia salina L.) bioassay is considered as a preliminary screening for the presence of antitumor compounds and used to determine the toxicity of plant extracts. Different extracts of three Egyp...
STUDY OF ANTIOXIDANT ACTIVITY OF FORMONONETIN BY IN VITRO METHOD
Objective: Now the important field of research in phytomedicine is to search a new plant source as well as new phytoconstituents that have fewer side effects and low cost with the free radical scavenging activity. Method...