Assessment for Performance of Middlebrook 7H9 with Oleic-Albumin-Dextrose-Catalase Culture Media for Isolation of Mycobacterium tuberculosis in Ziehl–Neelsen Smear-positive Sputum Samples in Clinically Suspected Cases of Tuberculosis
Journal Title: INTERNATIONAL JOURNAL OF SCIENTIFIC STUDY - Year 2019, Vol 6, Issue 11
Abstract
Introduction: Pulmonary tuberculosis (TB) is a second foremost cause of death from a communicable disease, after the HIV. Being communicable should be diagnosed at the earliest. Smear examination is preliminary step for the confirm diagnosis, but culture is still a gold standard method. Materials and Methods: The present study was carried out in the Department of Microbiology on a total of 600 smear sputum samples from clinically suspected cases of pulmonary TB attending the outpatient and inpatient departments of MMIMSR, Mullana, Ambala, from December 2016 to June 2018. Specimens were subjected to ZN and LED staining before and after decontamination. After microscopy, specimens were subjected to culture on LJ and Middlebrook 7H9. Results: Mycobacterium tuberculosis was isolated in 23.33% of samples. 110 (78.57%) were detected by microscopy (ZN and LED), respectively. ZN smear positivity before and after decontamination was maximum in mucopurulent 78% and 76.63% and LED 73.63% and 72.03%. Culture positivity on Middlebrook 7H9 was 100% while 87.85% on LJ media. The rate of contamination was 5% and 7% on Middlebrook 7H9 and LJ media, respectively. Conclusions: Middlebrook media was superior to the conventional LJ medium in being rapid, easy to use and interpret, and significantly low time-to-growth detection and had lesser contamination rate because the liquid media contains growth supplement oleic-albumin–dextrose-catalase, provides additional nutrition.
Authors and Affiliations
Nitika Saini, Rajendra Kumar Saini, Varsha A Singh
Keywords
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