Assessment of Cytotoxicity of Titanium Tetrafluoride on Human Fibroblast Cell Line and Optimisation of Operational Conditions using Response Surface Methodology
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 12
Abstract
Introduction: Fluoride products have an important effect on preventing Early Childhood Caries (ECC). Titanium tetrafluoride (TiF4)’s efficacy as a preventive agent suggests its use for inhibition of caries in man; however, its cytotoxicity is still questionable. Aim: To assess the optimisation of operational conditions via detecting the cell viability against TiF4. Materials and Methods: In order to find the optimal operational conditions after TiF4’s application on human Gingival Fibroblastlike cells (HGF1-PI 1), the Design of Experiments (DoE) based on Central Composite Design (CCD) was utilised. Concentration, pH and time of application of TiF4 solution were the three factors that were evaluated for optimisation of operational conditions. Results: The proposed optimal operational condition included 2.28% concentration of TiF4 solution with pH value of 1.89 and 1.09 minutes of application period. The model prediction of 86% cell viability at this optimal condition was verified experimentally at 82% of cell viability, presenting 95.34% of agreement. This consistency of the values proved the precision of the Response Surface Methodology (RSM) method to predict optimal operational conditions. Conclusion: The study introduced RSM as a precision and valid method to evaluate cytotoxicity of newly-developed dental materials. DOE-based studies could be successfully employed for optimising the operational conditions during TiF4 solution application to minimise the possible toxicity on fibroblastlike cells. Since the present proposed optimum conditions are in 95.34% of agreement with the experimental ones, thus these conditions can be considered as optimum operational conditions.
Authors and Affiliations
Tahereh Eskandarian, Mohammad Hossein Morowvat, Mahsa Zare
Keywords
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- EP ID EP368130
- DOI 10.7860/JCDR/2017/29838.11000
- Views 58
- Downloads 0
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