ASSESSMENT OF DISINFECTION AND CLEANING VALIDATION IN CENTRAL LABORATORY, MBS HOSPITAL, KOTA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 10
Abstract
BACKGROUND Many outbreaks are still caused by contaminated surfaces, and the technical drawbacks of liquid disinfectants may be partially to blame for the high facility-acquired infection rates in health care. These outbreaks have been reported when auxiliary hospital staff members have not followed appropriate protocols and have lacked supervision by Infection Control Committee. MATERIALS AND METHODS A total of 36 samples were collected by sterile swab sticks. From each site, three sets of samples were collected by the sterile swab sticks, the first sample being after the day’s work was completed, the second after cleaning had been done the next day and the third after cleaning was correctly completed under supervision. All samples were collected from different sites (Laboratory slab, floor, test tubes and serology discarding jars). The swabs were dipped in thioglycolate broth after collection, from where it was later plated on standard plates by lawn culture after standardisation on McFarland 0.5% BaSO4. Colonies of growth were counted by colony counter. All results were calculated in logarithm. RESULTS After disinfecting with non-calibrated disinfectant without specified contact time, there was unsatisfactory log reduction found for various microorganisms. When all disinfectants were used in calibrated and prescribed manner with all SOPs having been followed, there were satisfactory results. A careful assessment of its physical area is needed with a view to improvement and the installation of adequate resources to carry out techniques in material traceability and professional and environmental safety. Protocols, procedures and written routines need to be validated in terms of applicability and to be readily available in each area depending on its specific characteristics. CONCLUSION Most disinfectant program failures occur either because the wrong chemistry disinfectant was selected or because the right chemistry is not applied properly as per SOP. A careful assessment of its physical area is needed with a view of improvement and the installation of adequate resources to carry out techniques in material traceability and professional and environmental safety.
Authors and Affiliations
Seema Malav, Naveen Saxena
Keywords
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- EP ID EP411282
- DOI 10.14260/jemds/2018/287
- Views 53
- Downloads 0
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