Assessment of Microbiological Profile of Bile in Cholecystectomy Patients: A Microbiological Study
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 5
Abstract
Background: Calculus disease of biliary is one of the most common disorders affecting the gastrointestinal tract constituting a major cause of morbidity. Antimicrobial therapy recommendations state that antibiotics should be administered as soon as the diagnosis of acute cholangitis is suspected or established. Hence; we planned the present study to assess the microbiological profile of bile in cholecystectomy patients. Materials & Methods: A total of 50 laparoscopic cholecystectomy (LC) patients were included in the present study. 4 cubic cm of bile was aspirated with use of sterile 20 No. spinal needle with 10cc sterile syringe in laparoscopic cholecystectomy. This collected bile from gallbladder during cholecystectomy was transported to the microbiology laboratory in sterile vial. Inoculation of bile for a culture in culture media like brain heart infusion agar, Mac-Conkey agar and blood agar, was done and identification of the organism was done using different biochemical tests. Results: Escherichia coli, Enterococcus spps., Staphylococcus aureus, Salmonella spps., and Pseudomonas spp. were the common microorganisms observed in the bile samples in the present study. Conclusion: Bile infection often complicates gall stone diseases.
Authors and Affiliations
Ravi Kumar, Amit Kumar Anand
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