ASSESSMENT OF PHYLOGENTIC RELATIONSHIP OF WHEAT CULTIVARS USING MICROSATELLITE MARKERS
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 52
Abstract
In present study Genetic diversity of the ten wheat varieties were analyzed at the DNA level using 14 wheat specific microsatellite markers. 12 markers out of 14 had detected 58 alleles with an average of 4.8 alleles per primer. The resolving power (Rp) of each primer ranged from 1.6 for the SSRW3 to 7.2 for SSRW2 primer. The gene diversity based on microsatellite markers was ranged from 0.24 to 0.97 with an average value of 0.66. The genotypes V-23 and VWTH-08-07 showed the maximum genetic similarity 0.66 out of all 10 wheat varieties. The genotypes HUW-312 shows maximum genetic diversity with HD-2133 and HUW-825. The range of genetic diversity values broadly indicates the degree of heterogeneity or homogeneity in different genotypes of the plant species. As a result of this study, genetically diverse parents can be identified and also indicates that microsatellite markers could characterize and discriminate varieties from each other.
Authors and Affiliations
NARESH PRATAP SINGH
Keywords
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