STANDARDIZATION OF MEDIA COMBINATIONS FOR HARDENING OF ORCHID DENDROBIUM Spp Cv. SONIA-17
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 6
Abstract
The present investigation was carried out on “standardization of media mixture for hardening of in vitro plantlets with an objective to develop protocol for hardening of the In-vitro grown Dendrobium cv. Sonia 17 with 8 different treatment combinations. Viz. T1-cocopeat; T2-cocopeat +1pc coconut husk; T3-cocopeat +2pc coconut husk; T4-cocopeat +3pc coconut husk; T5-cocopeat + charcoal (1: 1); T6-cocopeat + charcoal + 1pc coconut husk; T7-cocopeat + charcoal + 2pc coconut husk; T8-cocopeat + charcoal + 3pc coconut husk. Treatments consisting of plantlets 3 pieces coconut husk tied with the ex-agar plantlets and planted in the pro-tray containing coco peat (T4) significantly enhanced the formation of new leaf/plantlet (5.3)no of roots/plantlets(7.5), length of the root (2.5 cm) and percentage of survival (96.67%) of the plantlets. While lowest values in terms of new leaf/plantlet (4.17 )no of roots/plantlets(6.33 ), length of the root (1.83 cm) and percentage of survival (53.33%) are obtained from treatment consisting of coco peat media only.
Authors and Affiliations
S. MUNA
Keywords
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