Assessment of survival and ploidy condition of Zebra fish progeny produced through androgenesis using Disperms
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 4
Abstract
Over exploitation, habitat modification, pollution load and introduction of exotic species affect fish genetic diversity directly or indirectly. Excessive mortality of fishes due to any of these factors lead to species reduction of population size or extinction. Hence conservation of aquatic genetic resources is essential to maintain ecological as well as socioeconomic equilibrium. Ex situ strategies like cloning of fish gametes and embryos are proven globally important. In conventional methods, production of androgenetic clones was done using X rays or γ rays followed by subjecting the eggs to thermal/ hydrostatic/ pressure shock for diploidization after monospermic activation. Due to two subsequent treatments of radiation and shock, the survival rates of putative androgenotes are presumed to be very low. The present study is carried out to assess the survival and ploidy condition of diploid androgenetic progeny of zebrafish, Danio frankei, produced through dispermic fertilization. Poor survival rates of hatchlings produced through fertilization of genome inactivated eggs with monosperms compared to the survival ability of hatchlings produced through fertilizing genome inactivated eggs with disperms was discussed. This study clearly showed that the use of combination of Poly Ethylene glycol (PEG) and Calcium chloride (CaCl2) is more effective than any single agent to form viable disperms that can induce fertilization of genome inactivated eggs similar to the effect shown by normal eggs.
Authors and Affiliations
D Sai Vinathi, V Sridevi, DC Reddy
Keywords
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