Association between HLA-A, HLA-C and HLA-B Genes and Ankylosing Spondylitis in Macedonian Population
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2012, Vol 5, Issue 3
Abstract
Aim: The aim of this study was to determine the association of HLA-A, -C and -B genes with ankylosing spondylitis in patients from the Republic of Macedonia. Material and Methods: This study included 307 subjects (250 healthy individuals and 57 patients with ankylosing spondylitis who were diagnosed at the University Clinic of Rheumatology in Skopje). The HLA typing of class 1 (HLA-A, HLA-C and HLA-B) genes was performed using the method of Reverse Line Strip, after isolation of DNK from the blood leucocytes with the standard phenol-chloroforme method. The HLA sub typing of HLA-B*27 was performed with high resolution single-strand polymorphism. Results: We found negative association between HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18, as well as positive association between HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles and patients with ankylosing spondylitis. Both HLA-B*27:02 and HLA-B*27:05 alleles have shown positive association with ankylosing spondylitis. Conclusion: Group specific alleles HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18 are significantly more frequent in healthy individuals than in patients with ankylosing spondylitis which suggests their protective role. HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles (HLA-B*27:02 and HLAB*27:05) have strong positive or susceptible association with ankylosing spondylitis.
Authors and Affiliations
Meri Kirijas| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Snezhana Mishevska-Perchinkova| University Clinic of Rheumatology, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Anzelika Karadzova-Stojanoska| University Clinic of Rheumatology, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Olivija Efinska-Mladenovska| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Aleksandar Petlichkovski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Dejan Trajkov| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia, Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Cyril and Methodius”, Skopje, Republic of Macedonia
Keywords
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- EP ID EP8871
- DOI 10.3889/MJMS.1857-5773.2012.0255
- Views 357
- Downloads 17
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