Association of 10 p Locus with Slovenian Inflammatory Bowel Disease Patients
Journal Title: Annual Research & Review in Biology - Year 2015, Vol 8, Issue 3
Abstract
Aim: To evaluate association of 10 p locus in Slovenian inflammatory bowel disease (IBD) patients with different sub-phenotypes. Methodology: Genotyping of three selected SNPs from 10 p locus was performed in 594 IBD patients, divided into three sub-phenotypes, Crohn’s disease (CD), ulcerative colitis (UC) and refractory CD, and in 250 healthy controls with PCR-RFLP technique. Clinical characteristics of patients were compared according to genotype of selected SNPs. Results: We found statistically significant correlation of all three selected SNPs with different sub-phenotypes of IBD. For SNP rs12777960 on gene CCNY we found association with all IBD patients and UC patients separately, where frequency of AA and AC genotype was higher in a group of patients compared to controls (P = .007). For SNP rs4934697 we found statistically significant association only for refractory CD patients, where frequency of CT and TT genotype was higher in a group of patients compared to controls (P = .01). For SNP rs2254252 on gene SEPHS1 we found association for a group of all IBD patients and CD patients, where frequency of allele T was higher in a group of patients (0.521) compared to healthy controls (0.451, P = .009). We identified novel association between age at diagnosis and genotype of SNP rs12777960 in gene CCNY where patients with disease susceptible genotype AA were diagnosed younger compared to patients with AC/CC genotype (P = .03). Conclusion: We confirmed candidate SNPs and genes from 10 p region in population of Slovenian IBD patients and found specific correlations of SNPs with different sub-phenotypes of IBD, particularly SEPHS1 with CD, CCNY with UC and region near CREM and CCNY genes with refractory CD.
Authors and Affiliations
Katja Repnik, Mario Gorenjak, Uroš Potočnik
Keywords
Related Articles
- EP ID EP353477
- DOI 10.9734/ARRB/2015/20566
- Views 90
- Downloads 0
Validity and Reliability of Combination of Observed Practice with Objective Structured Clinical Examination (OSCE) in Midwifery Final Exam
Objective: The assessment of midwifery competency in final examination is the one of the most important tests in midwifery educational course of students in bachelor’s degree. As objective structured clinical evaluation...
Time-kill Study of Ethyl Acetate Extract of Stinging Nettle on Bacillus subtilis subsp. spizizenii ATCC CRM-6633 Strain NRS 231
Aims: This work investigated the antibacterial activity of selected ethyl acetate extract of Urtica dioica against Bacillus subtilis subsp. spizizenii ATCC® CRM-6633™ Strain NRS 231 (subtilin producer) based on the time-...
A Taxonomic Revision of Genus Polygonum L. sensu lato (Polygonaceae) for Flora of Iran
The genus Polygonum L. sensu lato is revised based on articles, herbarium documents and field observations. In this article, a brief revision of this genus in Iran is presented. It was first described by Rechinger and Sc...
Placenta Development and Ki-67 Nuclear Immunolocalization in Placental Tissues of the Wild Type and Domesticated Silver Fox (Vulpes fulvus Desm.)
Aims: To study development of placenta in wild type and domesticated silver fox with special reference to cell cycle progression of the invasive trophoblast cells. Study Design: Immunohistochemistry with cytokeratin and...
Comparative Study between Greeks and Albanians with the Use of Restriction Fragment Length Polymorphisms of Apolipoprotein B Gene
Aim: The aim of this study was to compare the genetic polymorphisms of apolipoprotein B gene between two different populations, Greeks and Albanians living in Greece and to investigate the possibility of discriminating t...