Association of Serum Free Haemoglobin in Normoglycemic and Hyperglycemic Individuals
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 6
Abstract
ABSTRACT Introduction: Besides glycation induced injury, hyperglycemia in both type 1 and 2 diabetes can also increase oxidative stress leading to alterations in erythrocytic structural-functional organisation, shortening of life span and eryptosis. Free Haemoglobin (fHb) is released in circulation that could cause deleterious clinical effects. No study has associated fHb with hyperglycemia till now, thus it is pertinent to examine this aspect. Aim: The present study has associated serum fHB with hyperglycemia by simple, rapid, inexpensive spectrophotometric assay using Allen correction. Materials and Methods: The present case control study consisted of a total of 74 participants grouped into normoglycemic controls (n=37) and hyperglycemic subjects (n=37). Serum sample were analysed for haemoglobin (Hb) concentration by direct spectrophotometry using Allen correction. Concentration of Hb was calculated by Harboe’s Allen correction as Hb (mg/L)=1.68.A415-0.84.A380-0.84.A450 (absorbance in milliabsorbance). Statistical analysis was done using Statistical Package for Social Sciences (SPSS) version V21.0 and Microsoft excel software employing independent unpaired student’s-t-test and pearson’s correlation coefficient and χ2 test for trend while Odds Ratio (OR) and corresponding 95% CI was calculated using binary logistic regression. Results: Mean serum fHb levels were significantly elevated in hyperglycemia (101.74±61.73 mg/L; p=0.001) as compared to controls (58.44±39.69 mg/L). A dose dependent relationship was evident between increased serum fHb with hyperglycemia (p<0.001) and OR was statistically significant in hyperglycemia [OR (95% CI)=2.95 (1.05–8.31); p=0.04]. Serum fHb concentration was positively and significantly correlated with fasting plasma glucose (FPG) (r=0.34, p=0.003). Conclusion: Hyperglycemia causes glycation of tissue and circulating proteins as well as oxidative stress to erythrocyte membrane leading to alterations in erythrocytic membrane fluidity and shortening of life span. Serum fHb is widely used as marker of intravascular haemolysis and fHb is estimated by immunoassay that is expensive and cyanide based spectrophotometry that is toxic. The current study has associated fHb with hyperglycemia with direct spectrophotometry using Allen correction that uses minimal reagents and is non-toxic besides being inexpensive. We found that levels of fHb were significantly increased in hyperglycemia than controls (p<0.05) and fHb was positively and significantly correlated with FPG (p=0.003). The findings of our study suggests that erythrocytes are susceptible to hyperglycemic injury and fHb could be estimated inexpensively as marker of haemolysis.
Authors and Affiliations
Jiut Ram Keshari, Manish Mandal, Montosh Chakraborty, Debajit Bagchi, Praveen Sablania
Keywords
Related Articles
- EP ID EP604777
- DOI 10.7860/JCDR/2019/41427.12897
- Views 132
- Downloads 0
A Prospective Study to Analyse the Agreement between Empirical Antimicrobials used in Infective Endocarditis and Culture Sensitivity Reports
ABSTRACT Introduction: Infective Endocarditis (IE) is defined as a microbial infection of the heart valve or the mural endocardium. Annually the incidence of IE is between 2 and 6 per 100,000 individuals. The diagnostic...
Vaginoplasty: An Innovative Method of Making the Mould
ABSTRACT Introduction: Vaginal agenesis is a rare congenital malformation of female genital tract. It is often associated with other organ anomalies. The patient generally presents with primary amenorrhoea in late pubert...
Additional Virtual Reality Sitting Balance Training Using XBox Kinect™ in Patients with Neurological Disorders: A Pilot Study
Introduction: Sitting balance is a prerequisite to upper extremity function, standing and walking, which is affected in various neurological diseases. It is important to attain a good level of sitting balance before one...
Scrub Typhus Complicated by Acute Respiratory Distress Syndrome and Haemophagocytic Lymphohistiocytosis in a Young Patient
ABSTRACT Scrub typhus is an endemic rickettsial disease causing vasculitis involving the small vessels of the body. Haemophagocytic Lymphohistiocytosis (HLH) is a hyperinflammatory syndrome characterised by excessive unb...
An Additional Visceral Impression on Spleen – A Rare Anatomical Scenario
An accessory spleen is a small mass of splenic tissue, found apart from main body. An unexpectedly rare location of accessory spleen in phrenicocolic ligament was encountered during the course of preclinical cadaveric di...