Autologous Cultured Chondrocytes Impregnated in Three-dimensional Biodegradable Scaffold for Chondral Defects in Rabbits – An Experimental Study
Journal Title: Journal of Bone and Joint Diseases - Year 2018, Vol 0, Issue 0
Abstract
Background: Osteochondral lesions in young adults, if left untreated invariably progresses to degenerative joint disorders. e problem adds a burden on finances and average productivity of the patients and countries’ economics. Among the known strategies for treatment, none has proven to be gold standard, and each one is having their own pros and cons. Osteochondral tissue engineering (OTE) offers a promising future where cultured chondrocytes can be grown on biocompatible biomaterial scaffolds and implanted on defects. Materials and Methods: We tested a novel three-dimensional scaffold using chitosan and poly-L-lactide (PLLA) in recommended proportion on animal experimental model. It was seeded with autologous chondrocytes harvested from tibial condyle of rabbits. 10 rabbits were implanted with this cell-seeded scaffold and were followed at 4, 8, and 12 weeks before being sacrificed. Histological and gross examination was graded on a rank scale. It was compared between the implanted knee and contralateral control void. Values were examined statistically. Results: Outcome shows good quality of cartilage tissue, less fibrotic growth, beer amalgamation with the surrounding tissue, uptake at the implanted bed, and regular proliferative growth as early as 8 weeks. e scaffold imparts structural stability to the chondrocytes. The scaffold does not interfere with the normal healing process and was less inflammatory by 12 weeks. Conclusion: OTE with bioscaffold using PLLA-chitosan and cultured chondrocytes has positive and promising outcomes in healing of cartilage defects.
Authors and Affiliations
Shivam Sinha, Abhimanyu Madhual, Amit Rastogi, Pradeep Kumar Srivastava, Sarada Prasanna Mallick
Keywords
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