Bacterial Isolates from Seafood in Scotland
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 2
Abstract
Vibrio spp., Enterobacteriaceae representatives and Listeria monocytogenes were isolated from commercially prepared smoked and fresh Atlantic salmon, smoked and fresh haddock, live mussels and oysters. Isolates were identified phenotypically and by sequencing the 16S rDNA gene. Vibrio spp. occurred in high densities (>106 CFU gˉ1 ) in mussels. Enterobacteriaceae representatives were recorded at 2.2 x 106 CFU gˉ1 and 2.0 x 106 CFU gˉ1 in fresh salmon and smoked haddock, respectively. Total heterotrophic counts in fresh salmon, live mussels and oysters reached 107 , 107 and 106 CFU gˉ1 , respectively. Listeria monocytogenes was recorded at 5.0 x 104 CFU gˉ1 in mussels. The prominent characteristics of fish spoilage isolates were demonstrated by the ability of the isolates to reduce trimethylamine oxide (TMAO) to trimethylamine, and to produce H₂S. The spoilage organisms revealed the ability to produce hydrolytic enzymes.
Authors and Affiliations
Ngozi Izuchukwu
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