Direct Allergy Test (DAT) – New method for detection of antigenantibody reactivity
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 6
Abstract
An alternative for the tests of immunodetection diagnoses is proposed in this work, based on the elaboration of a qualitative method that can aid in the identification of antigen-antibody immunochemical reactivity in rapid diagnoses of allergies. For this purpose, the allergen-containing Dermatophagoides farinae mite extract was immobilized with glutaraldehyde on reusable support and blocking steps, addition of antibody containing serum to the allergens present in the mite extract. The reactivity response was obtained after colorimetric revelation. The appearance of the purple color signaled the reactivity between antigen-antibody on the plate. The colored area was photographed and the reactivity was represented by the intensity of color in pixels using the gel analyzer software. The method was evaluated by repeatability tests, negative assay and variation in protein content. From the proposed method a positive reactivity response was verified for the serum of individuals sensitized by mite. The method showed better performance for application of the protein load of 500 μg of the mite extract, 0.50% (v/v) glutaraldehyde and 0.25% (w/v) blocking. Repeated analyzes performed on different days showed that the method is reproducible being in agreement when compared to the immunoblotting technique. The results obtained for the development of the antigen-antibody detection method showed its promising potential as an alternative aid in the screening of antigen-antibody allergic reactions. The qualitative method, however, does not aim at replacing traditional techniques, but it provides speed and cost reduction, as it eliminates the electrophoretic separation step, dispenses standard purified proteins, and reuses the support, besides providing less reagent consumption.
Authors and Affiliations
Francisca das C. S. Silva, Patrícia R. Pereira, Luciana D. de C. Girão, Anderson B. Matos, Maria C. Queiroz
Keywords
Related Articles
- EP ID EP422643
- DOI 10.9790/3008-1306022834.
- Views 68
- Downloads 0
Isolation, Characterization and Biological Activities of Antibacterial Antibiotics Produced by Streptomycesalbus Dsm 40313
This research was carried out in the search for bioactive substances from Streptomyces that demonstrated inhibitory effect against some pathogenic bacteria. Twelve Streptomyces strains were isolated from soil samples col...
Phytochemical Compositions of Fruits of Three Musa Species at Three Stages of Development
This was designed to evaluates the phytochemical compositions of fruits of three Musa species at three stages of development. Spectrophotometric methods were used for the study. Results of the qualitative phytochemical a...
Biological Issues and Distribution of Mongolian Gerbil (Meriones Unguiculatus, Milne Edward, 1876) in Mongolia
Grasslands are the dominant landscape in Mongolia, accounting for 70-80 % of the national land area.Spreading of Mongolian gerbil distribution throughout the Mongolia including sandy desert, sandy steppe, steppe and fore...
Study of Air quality Status of some cities of Rajasthan
An attempt has been made in this investigation to ascertain air quality on major roads of Ajmer city in the form of Air Quality Index ( AQI). Monitoring stations were set up at fifteen strategic locations on all arterial...
Hepatoprotective Activities of Girang Leaf Ethanol Extracts (Leea angulata Korth. Ex Miq) on Wistar Rats Induced by Paracetamol
Paracetamol is an analgesic and antipyretic drug that has a toxic effect when it is used with excessive doses. The toxicity of paracetamol is caused by the formation of NAPQI reactive metabolites and paracetamol inducing...