Bacteriological and Genetic Study of E-coli Isolated from different Infections in Diyala
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
The goal of present study was to Isolation and Identification of Escherichia coli from different Infections and detection the sensitivity and resistance of Ecoli to antimicrobials Additionally detection of virulence factors . This study was conducted from the period from 1 / 6 / 2016 to 1/ 9 / 2016 in Baquba city in Iraq.. It included; Fiftin samples were collected from different infections from Baquba General Hospital and AL-Batool Hospital. Twenty isolates were found to be Escherichia coli .The susceptibility test was applied on these isolates against deferent antibiotics. The results revealed that the highest resistances were for Piperacilline(93.3%) and highest Sensitive Imipenem with 100%, While the lowest resistance were for Tobramycin (9%) . Moreover the results of virulence factors that had E. coli showed possession of all isolates many virulence factors and a high production of which increases the pathoginicity of it. All isolates were unable to produce urease and gelatinase, but heamolycin (35%) . As well as, Tow isolate (10%) were able to production Extended Spectrumβ-Lactamases enzyme. Furthermore, four isolate (20%) were able to production metalloβ-Lactamases Finally, six isolate(30%) were able to production Bacteriocin. Conclusion: E. coli isolates highest resistances were for Piperacilline and highest Sensitive Imipenem with 100%, While the lowest resistance were for Tobramycin . additionally some of isolates production Extended Spectrumβ-Lactamases enzyme, metallo β-Lactamases and Bacteriocin.
Authors and Affiliations
Assistant lecturer Eman Abass Ali*, Assistant lecturer Ibtihal Hameed Mohsin
Keywords
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- EP ID EP387195
- DOI 10.9790/3008-110606108112
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