Behavior of Banana Striatum Virus in in vitro propagated plants of Musa hybrid cultivars FHIA 20 and FHIA 21
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The losses taken place by the black and yellow Sigatoka have impelled the introduction to the country of the tetraploid hybrids from FHIA, however, at the present time, the high incidence of Banana Streak Virus in these hybrids is well known. To evaluate the effect of the micropropagatión process, on the fluctuations of the viral concentration in the tissue, they were introduced to the in vitro propagation positive and negative plants serologically tested of the cultivars FHIA 20 and 21, previously indexed in field (DAS-ELISA), later on they passed to phase of adaptation to evaluate the derived results of the diagnosis tests carried out after 3-6 months of transplanted. As a result of the DAS-ELISA carried out to the plants in phase of adaptation (37) 22.2 % of the negative plants, indexed in field, was positive, while 68.4 % of the positive plants, indexed in field, was negative, in both cases all the plants were positive to the diagnosis for PCR. In some cases, given the implication of the obtained results, was used the ISEM as technique of diagnostic, for the corroboration of the same ones.
Authors and Affiliations
Orelvis Portal1*, José E. González2, Ricardo Hernández3, Mileidy Cruz1, Neyda Bacallao1, Tatiana Pichardo1, Ana L. Darías1, Leyanis García1 y Elio Jiménez1. * Autor para correspondencia. 1 Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las Villas. Carretera a Camajuaní km 5. 5. Santa Clara, Villa Clara, Cuba. e-mail:
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