In vitro response of rice varieties to water stress conditions
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The present study was carried out wth the objective of determining in vitro response of two rice varieties to water stress conditions of water stress using concentrations of Polyethyleneglycol – 6 000 (PEG). As basal medium in calli formation and plant regeneration the salts Murashige and Skoog were used (MS). In the first case, plant growth regulators as 2,4 - Dichlorophenoxyacetic (2,4-D) and 6 - Bencilaminopurine (BAP) were added to culture mediun, meanwhile for the second, 6 - Bencilaminopurine and Kinetin (Kin). In both, different concentrations of stressing agent were used, (0, 5.0, 7.5 and 10%), being determined their effect on osmotic potential, induction and growth of calli, as well as, plant regeneration. A differentiated behavior of varieties not only in calli formation and also in fresh and dry weight was evidenced in correspondance of its tolerance grade using 5 and 7.5% of PEG-6 000. Those concentrations favored shoot and plants induction in plant regeneration medium without the presence of PEG-6 000; meanwhile when itn was added to this kind of culture medium it inhibited this process completely.
Authors and Affiliations
Aymara García , María T. Marrero, María C. González
Keywords
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