In vitro response of rice varieties to water stress conditions
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The present study was carried out wth the objective of determining in vitro response of two rice varieties to water stress conditions of water stress using concentrations of Polyethyleneglycol – 6 000 (PEG). As basal medium in calli formation and plant regeneration the salts Murashige and Skoog were used (MS). In the first case, plant growth regulators as 2,4 - Dichlorophenoxyacetic (2,4-D) and 6 - Bencilaminopurine (BAP) were added to culture mediun, meanwhile for the second, 6 - Bencilaminopurine and Kinetin (Kin). In both, different concentrations of stressing agent were used, (0, 5.0, 7.5 and 10%), being determined their effect on osmotic potential, induction and growth of calli, as well as, plant regeneration. A differentiated behavior of varieties not only in calli formation and also in fresh and dry weight was evidenced in correspondance of its tolerance grade using 5 and 7.5% of PEG-6 000. Those concentrations favored shoot and plants induction in plant regeneration medium without the presence of PEG-6 000; meanwhile when itn was added to this kind of culture medium it inhibited this process completely.
Authors and Affiliations
Aymara García , María T. Marrero, María C. González
Keywords
Related Articles
- EP ID EP684854
- DOI -
- Views 131
- Downloads 0
Germination of somatic embryos of Psidium guajava L. cv. Cuban Red Dwarf EEA 18-40 in temporary immersion systems
Somatic embryo germination of Psidium guajava L. cv. Cuban Red Dwarf EEA 18-40 in temporary immersion systems (TIS), in which somatic embryos were cultured in the heart-torpedo stage in MS mediun at mayor half strength...
Influence of the time of year on the development of symptoms in ‘Grande naine’ plants artificially inoculated with Mycosphaerella fijiensis in a cultivation house
Plant pathogen fungi required favorable conditions to invade and colonize plant tissues, which may change according to the season of the year. This work is aimed to evaluate the influence of different seasons (June- Au...
Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse
Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on th...
Scaling-up the biomass production of Cymbopogon citratus L. in temporary immersion system
Shoot-tips, collected from greenhouse-grown plants of Cymbopogon citratus L. (lemmon grass), were incubated on a semi-solid Murashige and Skoog (MS) medium with 30% (w/v) sucrose, and supplemented with 0.89 μM 6-benzyl...
In vitro propagation of Cubans varieties of Solanum tuberosum L. ‘Yuya’, ‘Marinca’, ‘Grettel’ and ‘Ibis’
The in vitro culture techniques are used to support the production processes of potato seed (Solanum tuberosum L.) because it ensure the rapid propagation of promising varieties, genetic stability and high quality plant....