In vitro multiplication of Macleania rupestris (Kunth) A.C. Sm. (Ericaceae)
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 4
Abstract
Macleania rupestris (Kunth) A.C. Smith (Ericaceae) is a plant native to the Andes, which produces edible fruits with great food and commercial potential. M. rupestris plants have been in vitro established from seeds of ripe fruits and to increase their number it is necessary to define the conditions of their multiplication. The objective of the investigation was to determine the effect of zeatin and kinetin to in vitro multiply M. rupestris plants. As a basal culture medium, Woody Plant (WP) was used. Treatments included the CIRGEBB culture medium (WP + 0.5 mg l -1 zeatin), A (WP + 0.5 mg l -1 kinetin), B (WP + 1.0 mg l -1 kinetin) and WP as control. The variables evaluated were explant height (cm), number of shoots and number of leaves, at 60 and 120 days of culture. The use of zeatin and kinetin in the WP culture medium allow in vitro multiplication of M. rupestris. However, the addition of zeatin favors the development of plants with phenotypic characteristics suitable for micropropagation. Based on the results, it is proposed to use the culture medium A (WP + 0.5 mg l -1 kinetin).
Authors and Affiliations
Antonietta Ornella Gutiérrez Rosati, Yngrid De la Cruz Chacón
Keywords
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