Effects of IAA and IBA on the in vitro rooting of stem cuttings of Sechium edule (Jacq.) Sw
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 1
Abstract
This investigation was carried out with the objective of evaluating the response of shoots of chayote [S. edule (Jacq.) Sw.] to the application of indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) on the in vitro rooting phase. The effect of three culture media, modified from the proposed of Murashige and Skoog (MS) was studied: 1- 65% MS salt base, 2- 65% MS salt base + 0.05 mg l -1 of indolebutyric acid (AIB) and 3- 65% MS salt base + 3.0 mg l -1 -3-indole acetic acid (IAA). The variables evaluated were: number of roots, length and number of shoots per in vitro seedling. The best result were obtained when using 65% MS salt base with 3.0 mg l -1 of IAA. Significant differences (P<0.05) among treatments containing growth regulators was obtained for the variables number of roots and length of in vitro plantlets. In the UNA-730 accession root induction was obtained in a 65% MS salt base culture medium without growth regulators; however, when IAA and IBA was added the induction was 100%. The differences identified in this work with regards to the root induction were probably the combined result of genotype and specific culture conditions.
Authors and Affiliations
José García García, Eduardo Salas Alvarado, José Azofeifa Bolaños
Keywords
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