Effect of IAA on in vitro rooting of Gigantochloa atter (Hassk.) Kurz.
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
Vegetative propagation of Gigantochloa atter (Hassk.) Kurz. It is difficult due to their handling, transportation and poor root development. The tissue culture techniques allow increasing the number of these plants in a short period of time. In order to determine the effect of indole acetic acid (IAA) three concentrations (15, 20 and 25 mg l -1 ) on in vitro rooting were tested and a control without growth regulator it was included. After 20 days of culture the number of plants with roots was quantified, the plant height (cm) and root length (cm) was measured. The plants were transferred to acclimatization phase and after 20 days of culture survival was determined. The results showed that the IAA influenced the in vitro rooting of G. atter. By adding to the culture medium IAA (20.0 mg l -1 ) the highest percentages of rooting (96.0%) and ex vitro (60%) were obtained survival.
Authors and Affiliations
Yudith García-Ramírez, Marisol Freire-Seijo, Blanca Pérez
Keywords
Related Articles
- EP ID EP683437
- DOI -
- Views 139
- Downloads 0
In situ genomic DNA extraction for PCR analysis of regions of interest in four plant species and one filamentous fungi
The extraction methods of genomic DNA are usually laborious and hazardous to human health and the environment by the use of organic solvents (chloroform and phenol). In this work a protocol for in situ extraction of ge...
Effect the position of the male flower bud on callus formation and embryogenic cell suspensions establishment of plantain cv. ‘FHIA- 21’ (Musa AAAB)
The somatic embryogenesis response is close linked with the development phase of the explant donor plant. Therefore, the objective of this work was to determine the effect of male flower position in the plant on the em...
Influence of growth regulators and physical state of the culture medium on the in vitro multiplication of Colocasia esculenta clone 'INIVIT MC2001'
To achieve adequate results in the in vitro multiplication of taro cultivars is required to standardize the culture conditions. The aim of this study was to determine the influence of growth regulators and physical stat...
Effect of the selective agents kanamycin and sulfadiazine on the somatic embryogenesis process in Coffea arabica cv. Red caturra
It was studied the effect of the kanamicin and sulfadiacin like selection markers in the first phase of the genetic transformation of coffee (Coffea arabica). Embryogenic cell suspensions of coffee of the variety caturra...
Propagation of Morus alba var. Creole with the use of temporary immersion systems
The mulberry (Morus alba L.) it is a forage plant of great economic importance. The present work was carried out with the purpose of to in vitro propagate the variety ‘Criolla’ via organogenesis with the use of Temporar...