Effect of IAA on in vitro rooting of Gigantochloa atter (Hassk.) Kurz.
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
Vegetative propagation of Gigantochloa atter (Hassk.) Kurz. It is difficult due to their handling, transportation and poor root development. The tissue culture techniques allow increasing the number of these plants in a short period of time. In order to determine the effect of indole acetic acid (IAA) three concentrations (15, 20 and 25 mg l -1 ) on in vitro rooting were tested and a control without growth regulator it was included. After 20 days of culture the number of plants with roots was quantified, the plant height (cm) and root length (cm) was measured. The plants were transferred to acclimatization phase and after 20 days of culture survival was determined. The results showed that the IAA influenced the in vitro rooting of G. atter. By adding to the culture medium IAA (20.0 mg l -1 ) the highest percentages of rooting (96.0%) and ex vitro (60%) were obtained survival.
Authors and Affiliations
Yudith García-Ramírez, Marisol Freire-Seijo, Blanca Pérez
Keywords
Related Articles
- EP ID EP683437
- DOI -
- Views 131
- Downloads 0
Use of stem sections from in vitro papaya plants (hybrid IBP 42-99) to obtain callus with embryogenic structures
Inside in vitro propagation via, the somatic embriogenesis offers possibilities of obtaining top volumes of production in a minor period of time and a lower cost, which are a method potentially more efficient than the re...
Induction of somatic embryogenesis in Cocos nucifera L. from immature inflorescences
Including all the methods of vegetative multiplication, the somatic embryogenesis is the most promising for coconut multiplication. Immature inflorescences of Coppery Malay from different ages trees were used. Three con...
Effect of foliar fertilization on Ananas comosus L. Merr. cv. ‘Cayena lisa’ acclimatization
The low survival and slow growth of in vitro pineapple plants (Ananas comosus L. Merr.) in acclimatization stage limit the use of biotechnological techniques for it propagation. The aim of this study was to determine t...
Effect of inoculation time on Agrobacterium-mediated transformation efficiency of Musa cv. ‘Grande naine’ (AAA)
Increasing inoculation time during Agrobacterium-mediated transformation has been shown to favour transformation efficiency in several plant species. The effect of inoculation time in combination with spermidine (Spd)...
Potato purple top, Lethal wilt of oil palm, and Papaya twisted neck syndrome: Phytoplasma-associated diseases in Ecuador
Phytoplasma are wall-less bacteria limited to the phloem vessels in higher plants. Diseases associated with phytoplasma, in the past, have not been a serious problem in Ecuador. Nevertheless, and for climate change eff...