Effect of IAA on in vitro rooting of Gigantochloa atter (Hassk.) Kurz.
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
Vegetative propagation of Gigantochloa atter (Hassk.) Kurz. It is difficult due to their handling, transportation and poor root development. The tissue culture techniques allow increasing the number of these plants in a short period of time. In order to determine the effect of indole acetic acid (IAA) three concentrations (15, 20 and 25 mg l -1 ) on in vitro rooting were tested and a control without growth regulator it was included. After 20 days of culture the number of plants with roots was quantified, the plant height (cm) and root length (cm) was measured. The plants were transferred to acclimatization phase and after 20 days of culture survival was determined. The results showed that the IAA influenced the in vitro rooting of G. atter. By adding to the culture medium IAA (20.0 mg l -1 ) the highest percentages of rooting (96.0%) and ex vitro (60%) were obtained survival.
Authors and Affiliations
Yudith García-Ramírez, Marisol Freire-Seijo, Blanca Pérez
Keywords
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