In vitro establishment of Erythroxilum echinodendron
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
In the areas of serpentines live wild species whose propagation for traditional roads is not possible because they present problems in the germination of the seeds under nursery conditions. The main objective of this work was to achieve the establishment in vitro of Erythroxilum echynodendron, in order to increase the number of available postures for the restoration in the administrative unit El Recreo located in the protected area Cubanacán. For the in vitro establishment of the seeds the effect of a Sodium Hypochlorite solution (2.0% NaOCl) during different times of disinfection (15, 20, 25 min.) was studied. Whole seeds and seeds which the embryonic sack with embryo inside (the pericarp and the endosperm were moved away) were sowed in the culture medium composed by 50% of the MS salts and 2.0% of sucrose. The bigger percentage of seeds free of microbial pollutants (60%) was achieved when the treatment with 2.0% of NaOCL during 25 minutes was used. The biggest percentage of germinated seeds (80.7%) was achieved when the embryonic sack with the embryo inside was sowed in the culture medium.
Authors and Affiliations
Elisa Quiala, Grecia Montalvo, Jesús Matos, Manuel de Feria, Reynaldo Mederos, Maité Chávez
Keywords
Related Articles
- EP ID EP690245
- DOI -
- Views 127
- Downloads 0
Influence of magnetic field on the in vitro establishment of zygotic embryos of Adenanthera pavinina L.
The in vitro embryo culture for pre germinal treatment allows the application of low frequency electromagnetic and magnetic fields. The species Adenanthera pavonina L. has seeds with a waterproof integument, long laten...
Effect of silver nitrate on the in vitro germination of Euphorbia nutans Lag.
Euphorbia nutans Lag. it is a herbaceous plant found in the Mixteca Poblana, Mexico. It is considered a weed, it is not cultivated and its medicinal use to treat gastric problems and heal wounds is ignored. This situatio...
Protocol for the isolation of intact chloroplasts from leaves Musa spp. obtained by in vitro culture and evaluation of photosynthetic activity
Chloroplasts are organelles of plant cells where photosynthesis takes place by converting light energy into chemical energy (ATP) and generating reducting power (NADPH). Isolation of chloroplasts and evaluation of phot...
In vitro establishment and multiplication of Guettarda clarense, endemic species of Cuba in danger of extinction
The present work was carried out with the objective of achieving the in vitro establishment and multiplication of Guettarda clarense which is an endemic local of Santa Clara city and it is considered in extinction dange...
Evaluation of phenotypic variability and early selection for rust of sugarcane (Puccinia melanocephala H. & P. Syd) in plants regenerated from irradiated calluses of the cane variety sugar ́SP 70-1284 ́
It was evaluated the variability fenotípic in early phases of selection from plants regenerated of the mutagenic treatment with dose of 30 Gy of radiations Gamma (source 60Co) applicated to calluses in growth of the dona...