In vitro establishment and multiplication of Guettarda clarense, endemic species of Cuba in danger of extinction
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
The present work was carried out with the objective of achieving the in vitro establishment and multiplication of Guettarda clarense which is an endemic local of Santa Clara city and it is considered in extinction danger. Seeds of mature fruits collected under field conditions were used. The effect of a concentration of NaOCl (2%) during 15, 20 and 25 minutes in the disinfection of the seeds was studied. The seeds were sowed in a culture medium of germination compound by 50% of MS salts. During the multiplication phase of the explants, the effect of three concentrations of 6-BAP (0.2, 0.4 and 0.6 mg. l -1 ) was studied. The culture medium was compound by the MS salts, 1.0 mg. l -1 of thiamine and 3% of sucrose. The bigger number of seeds free of visible microbial pollutants (50%) was obtained when the treatment with 2.0% of NaOCl during 25 minutes was used. The 85% of the seeds germinated in the culture medium compound by the 50% of the MS salts without growth regulators. During the multiplication phase of the explants, the multiplication coefficient and the longitude of the plants were increased in the same proportion as the concentration of 6-BAP was increased. The highest values were obtained (6.1 explants and 6.7 cm respectively) in the treatment with 0.6 mg. l -1 .
Authors and Affiliations
Elisa Quiala , Grecia Montalvo , Jesús Matos , Maité Chávez , Manuel de Feria , Reinaldo Mederos
Keywords
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