Evaluation of the effect of Pectimorf in the establishment and budding of axillary buds of sweet potato clone CEMSA 78-354 starting from buds
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 2
Abstract
With the purpose of studying the effect of different Pectimorf dose in the establishment and budding of axillary buds of sweet potato starting from buds, tuberous roots belonging to the clone CEMSA 78-354 were collected and placed in flasks with water in the laboratory under semicontrolled conditions to induce the sprouting of the buds. Later on, axillary buds of the buds of the tubers (establishment) and axillary buds coming from in vitro plants (multiplication)were selected as sowing material (explants), which were sowed in the Murashige and Skoog culture medium containing the MS salts, thiamin (1 mg.l -1 ), myoinositol (100 mg.l -1 ), sucrose (3%), gelryte (2 g.l -1 ), giberelic acid (10.0 mg.l -1 ) and indol-3-acetic acid (0.05 mg.l -1 ) and the already mentioned culture medium without the previously refered regulators of the growth with different Pectimorf concentration: 5.0, 10.0 and 15.0 mg.l -1 . The morphological behavior of the in vitro plantas was evaluated to the five, fifteen and thirty days after established the buds under in vitro conditions , and different variables related with the in vitro growth of the buds were analyzed, among which we have: number of sprouted buds, height of the in vitro plants (cm), number, longitude and width of the leaves, buds with roots, longitude of the roots (cm) and longitude of the petiole (cm). The best results were obtained when using the axillary buds coming from budding of tuberous roots in comparison with the axillary buds of the in vitro plants and with the use of the culture medium containing 10.0 mg.l -1 of Pectimorf.
Authors and Affiliations
Orlando S. González Paneque , Alejandro Falcón Rodríquez, Margarita Hernández Espinosa , Ramón Iglesias Curbelo, Juan J. Silva Pupo, Mirtha López Machado, Julio E. Rodríguez Hernández, Lizardo Arias Gómez, Juan C. Cabrera, Edubar Oliva Jaume
Keywords
Related Articles
- EP ID EP684325
- DOI -
- Views 146
- Downloads 0
Genetic transformation by Agrobacterium tumefaciens in papaya hybrid from in vitro plant shoot-tips
In papaya (Carica papaya L.) viral infections can decrease production by more than 50%. Genetic transformation consists of an alternative means of obtaining new varieties, which are resistant to this pathogen. The main...
In vitro multiplication of Paeonias sp. variety ‘SeSu’ in Temporary Immersion Systems
Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) a...
Effect of the intercellular extracts of banana leaves inoculated with Mycosphaerella fijiensis Morelet, on the electronic transport in chloroplasts of the cv Grande naine (AAA)
Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the ph...
Effect of inoculum density on the morphology and physiology of Bambusa vulgaris Schrad. ex Wendl shoots cultured in Temporary Immersion System
Bambusa vulgaris Schrad. Ex Wendl is a species of great environmental importance and plays a vital role in the economic development of Cuba. The present work was carried out with objective of determining effect of inoc...
Morphological characterization of Phaseolus vulgaris L. lines in greenhouse
Knowing and characterizing the preserved genetic heritage contributes to the development of new plant cultivars. The objective of the present work was to characterize morphologically, in greenhouse, lines of common bea...