Sporophyte regeneration of Platycerium bifurcatum (Cav.) C.Chr. from in vitro germinated spores
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 2
Abstract
In Cuba one of the most popular ferns, as an ornamental plant, is elkhorn (Platicerium bifurcatum) but its vegetative propagation is very slow so it is insufficient supply of this plant on the market. Besides, there is not in vitr o propagation methodology for this species. This work was developed with the aim of obtaining Platycerium bifurcatum sporophytes from germinated spores in culture media. Spores were obtained from mother plants fertile fronds o f P. bifurcatum. These were disinfected with different concentrations of sodium hypochlorite (1, 2, 3%) for 15 minutes. We evaluated the contamination percentage and spore germination. Additionally, we studied the MS salts concentration for the prothallus multiplication (0, 25, 50, 75, 100%) and we quantified sporophytes number by explant. Also we investigated the effect of the physical state of the culture medium for growth and sporophytes multiplication. With the results obtained it was defined that with 2% sodium hypochlorite and 15 minutes of exposure was reduced the percentages of contamination and was not affected spore germination. Most gametophytes multiplication achieved with the culture medium containing 50% MS salts, 1mg l -1 thiamine and 2% sucrose. The physical state of the culture medium did not influence the growth and multiplication of the sporophytes, which allowed select the liquid culture medium for their growth.
Authors and Affiliations
Lourdes R. García, Damaris Torres, Carlos Romero
Keywords
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