Influence of MS salts and sucrose concentrations of the culture medium for in vitro plants on the growth of bacterial contaminants from the sugarcane micropropagation
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The influence of MS salts and sucrose concentrations on the growth of four strain of bacterial contaminant isolated from multiplication stage and two from the rooting stage of the sugarcane micropropagation was determined. Independent experiments were carried out with each component in Petri dishes with the media for the multiplication and rooting of in vitro plants. Treatments were elaborated with different concentrations of salts MS expressed in percentage (p/v): 0, 25.0, 50.0, 75.0, 100.0, 150.0, 200.0, as well as of Sucrose: 0, 10.0, 20.0, 30.0, 40.0g.l-1. The Petri dishes were inoculated with 3.5μl of each bacterial suspension to a final concentration of approximately 10 4 cfu.ml -1 . The growing or not of the bacterial strains was evaluated at 24, 48 and 120h of incubation at 30oC and it was registered following the approach of: (+) growth on the inoculation place, (-) total absence of growth on the inoculation place. It was demonstrated that the salts and sucrose concentration in multiplication and rooting media of the in vitro plants limited the bacterial growth.
Authors and Affiliations
bacterianos de la micropropagación de la caña de azúcar Yelenys Alvarado Capó, Mileidy Cruz Martín, Nayanci Portal González, Mayra Acosta Suárez, Michel Leiva Mora
Keywords
Related Articles
- EP ID EP684855
- DOI -
- Views 136
- Downloads 0
Influence of the in vitro environment on the germination of somatic embryos of Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K.
The in vitro environment is a factor that in recent years has begun to investigate, because gases such as oxygen, carbon dioxide and ethylene play an important role in the morphogenesis of somatic embryos and their dev...
Callus formation of Persea americana Mill. Catalina cultivar starting from in vitro plant leaf segments
ABSTRACT The importance of establishing protocols for the regeneration of fruit-trees through out the organogenesis and the somatic embryogenesis is based on the possibility to use them in the propagation of the specie...
Applications of biotechnology to the propagation of papaya
The papaya (Carica papaya L.) is one of the most world wide consumed fruit. The use of the biotechnology as a tool in the micropropagation of elite plant in this specie can be an alternative to acelarate its multiplicat...
Potential of Trichoderma species as biofertilizer and biological control on Oryza sativa L. cultivation
Rice (Oryza sativa L.) is one of the main staples around the world, especially in Asia, the most consuming region. Rice cultivation currently faces many environmental problems caused by the contribution of chemical ferti...
Plant growth promoting rhizobacteria: potential for sustainable agriculture
With growing world population, there is an exponential increase in demand for food. The use of chemical fertilizers to improve crop productivity is associated with considerable health risks, environmental hazards, and co...