Influence of MS salts and sucrose concentrations of the culture medium for in vitro plants on the growth of bacterial contaminants from the sugarcane micropropagation
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The influence of MS salts and sucrose concentrations on the growth of four strain of bacterial contaminant isolated from multiplication stage and two from the rooting stage of the sugarcane micropropagation was determined. Independent experiments were carried out with each component in Petri dishes with the media for the multiplication and rooting of in vitro plants. Treatments were elaborated with different concentrations of salts MS expressed in percentage (p/v): 0, 25.0, 50.0, 75.0, 100.0, 150.0, 200.0, as well as of Sucrose: 0, 10.0, 20.0, 30.0, 40.0g.l-1. The Petri dishes were inoculated with 3.5μl of each bacterial suspension to a final concentration of approximately 10 4 cfu.ml -1 . The growing or not of the bacterial strains was evaluated at 24, 48 and 120h of incubation at 30oC and it was registered following the approach of: (+) growth on the inoculation place, (-) total absence of growth on the inoculation place. It was demonstrated that the salts and sucrose concentration in multiplication and rooting media of the in vitro plants limited the bacterial growth.
Authors and Affiliations
bacterianos de la micropropagación de la caña de azúcar Yelenys Alvarado Capó, Mileidy Cruz Martín, Nayanci Portal González, Mayra Acosta Suárez, Michel Leiva Mora
Keywords
Related Articles
- EP ID EP684855
- DOI -
- Views 139
- Downloads 0
Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the s...
Cardenolide metabolism and genetic transformation of Digitalis. Potentials and challenges
Cardenolides are secondary metabolites produced by plants of the genus Digitalis. These are widely used in treatments of congestive heart failure. Failed attempts to obtain suitable cardenolide levels from in vitro cul...
Designing a curriculum for Plant Biotechnology elective course for students in pre-university
To introduce the plant biotechnology in the curriculum of Vocational Pre-university Institutes of Sciences (IPVCE), increase the culture of the students in this specialty and motivate them to related careers, this stud...
Methodological procedure for conformation, implementation and improvement of quality systems for biofactories
There are a number of reports and references on quality, which broadly covered the concepts, bases, importance, techniques and methods, but only theorically. This issue enables the existence of quality systems limited t...
In vitro crop physiology
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features...