Influence of MS salts and sucrose concentrations of the culture medium for in vitro plants on the growth of bacterial contaminants from the sugarcane micropropagation
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 3
Abstract
The influence of MS salts and sucrose concentrations on the growth of four strain of bacterial contaminant isolated from multiplication stage and two from the rooting stage of the sugarcane micropropagation was determined. Independent experiments were carried out with each component in Petri dishes with the media for the multiplication and rooting of in vitro plants. Treatments were elaborated with different concentrations of salts MS expressed in percentage (p/v): 0, 25.0, 50.0, 75.0, 100.0, 150.0, 200.0, as well as of Sucrose: 0, 10.0, 20.0, 30.0, 40.0g.l-1. The Petri dishes were inoculated with 3.5μl of each bacterial suspension to a final concentration of approximately 10 4 cfu.ml -1 . The growing or not of the bacterial strains was evaluated at 24, 48 and 120h of incubation at 30oC and it was registered following the approach of: (+) growth on the inoculation place, (-) total absence of growth on the inoculation place. It was demonstrated that the salts and sucrose concentration in multiplication and rooting media of the in vitro plants limited the bacterial growth.
Authors and Affiliations
bacterianos de la micropropagación de la caña de azúcar Yelenys Alvarado Capó, Mileidy Cruz Martín, Nayanci Portal González, Mayra Acosta Suárez, Michel Leiva Mora
Keywords
Related Articles
- EP ID EP684855
- DOI -
- Views 132
- Downloads 0
Root length: morphological indicator of Phaseolus vulgaris L. response to hydric stress in greenhouse
Drought during the terminal phase of the crop is a global limitation for the production of common beans (Phaseolus vulgaris L.) and it is a problem that will be aggravated by the effects of climate change. The objectiv...
Study of genetic affinities in clones of Manihot esculenta Crantz of economic importance for Cuba
The genetic affinity were studied between 12 Cassava clones (Manihot esculenta Crantz) after studying the isozymatical systems: Peroxidase, Phenoloxidase and Carbonic Anhidrase. The MAT-GEN program was used for the analy...
Effect of Sodium Chloride on the in vitro development of cultivars 'Gran enano' and 'FHIA 03'
The effect of Sodium Chloride levels on in vitro development of Gran Enano (AAA) and `FHIA 03`(AABB) cultivars was determined. Researches were carried out at Instituto de Investigaciones en Viandas Tropicales (INIVIT)...
Effect of substrate type on the acclimatization of in vitro plants of Bambusa vulgaris
Bambusa vulgaris var. vulgaris has several uses. The biotechnological propagation can be an alternative to reach the demand of planting material. This investigation was aimed to determine the influence of substrate type...
Effect of filtered bacterial cultures with antifungal activity in vitro in the interaction Musa spp.- Mycosphaerella fijiensis
Although certain bacterial species may be in vitro antagonistic of Mycosphaerella fijiensis, as it was demonstrated, they are discrete results that have their effect on the Musa spp.-M. fijiensis interaction. The resul...