Determination of adequate concentrations of 2,4-D and 6-BAP for the induction of morphogenic calli of sweet potato
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 1
Abstract
Tubers from Inivit B 90-1 and Yabú-8, cultivars were put in the laboratories into water bottle and we chose explants of limbs from sprout. Explants were disinfected with Sodium Hypoclorite (Na OCl) with active chlorine (1%) and they were put into Murashige and Skoog culture medium under different concentrations of 2,4-D independent and combined with 6-BAP in the culture medium. Crops were put on the energy illumination. The best results in four weeks at the morfogenic callus induction were obtained with the concentrations of 2,4-D (0.1 mg.l-1) and 2,4-D (0.1 mg.l-1) combined with BAP (0.2 mg.l-1) in the culture medium.
Authors and Affiliations
Orlando S. González Paneque, Isabel Milanés Vega, Juan J. Silva Pupo, Angel Espinosa Reyes, Leonardo Acosta Pompa
Keywords
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