New protocol for the quick induction of adventitious buds and the regeneration of plants in banana cv. ‘Grande naine’ (Musa AAA)
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 1
Abstract
A protocol for the quick formation of adventitious buds in banana cv. ‘Grande Naine’ and the development of plants was developed. Several concentrations of 6-Benzylaminopurine (6-BAP) and 1-phenyl-3-(-1,2,3-thidiazol-5-yl)urea (TDZ) were studied in the culture media for the induction of these structures, evaluating some explants sizes for multiplication. The highest number of adventitious buds was obtained when TDZ was used in the culture medium, with only two subcultures needed to develop explants with adventitious buds. The explants of 1mm 3 were selected for the multiplication and a medium of MS salts with 1.0 mg.l -1 thiamine HCl and 2.0% sucrose for plant regeneration, reaching the values of 96%. The use of this protocol could be a very useful alternative in genetic improvement programs with the cv. ́Grande naine` using the mutation induction and somaclonal variation.
Authors and Affiliations
Lourdes R. García, J. P Pérez, Idalmis Bermúdez-Caraballoso, Pedro Orellana, Novisel Veitía, Leonardo García, Yenny Padrón, Carlos Romero
Keywords
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