In vitro antagonism of Trichoderma harzianum Rifai against Mycosphaerella fijiensis Morelet
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 4
Abstract
The in vitro antagonism of Trichoderma harzianum against Mycosphaerella fijiensis, foliar pathogen of banana and plantain, was evaluated. The assays were performed using the dual culture method. Competition for space and nutrients, the antagonistic capacity and forms and intensity of antagonism were determined considering the invasion of the surface of the colony, colonization and sporulation of T. harzianum on M. fijiensis after seven days of inoculation. Finally, the effect of volatile metabolites of T. harzianum was evaluated. The results showed in vitro antagonism of T. harzianum against M. fijiensis by competition for space and nutrients of the culture medium. Trichoderma grew over the pathogen colony with hyperparasitism and high intensity. Also, it completely covered the surface of the culture medium. T. harzianum not inhibited the growth of M. fijiensis by volatile metabolites. Damage was observed in the integrity of the cell wall of M. fijiensis hyphae and the cell content exit. The use of antagonistic fungi, could contribute to the design of strategies for integrated management of this disease.
Authors and Affiliations
Mayra Acosta-Suárez, Tatiana Pichardo , Berkis Roque , Mileidy Cruz-Martín , Eilyn Mena , Michel Leiva-Mora , Rosa Castro, Yelenys Alvarado-Capó
Keywords
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