Construction and partial sequencing from a subtractive library in cut Calcutta 4 ’(Musa AA) in early stage of infection with Mycosphaerella fijiensis Morelet
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 4
Abstract
The study of genes involved in plant defense response against pathogen attack, is one of most important steps leading to the elucidation of disease resistance molecular mechanisms. The generation of subtracted deoxyribonucleic acid libraries (cDNA), by means of suppression subtractive hybridization technique (SSH), has been used for this purpose. A subtractive hybridization was made between a cDNA population obtained from ‘Calcutta 4’ inoculated leaves with M. fijiensis (CCIBP-Pf83) and a mixture of cDNA from ‘Calcutta 4’ non inoculated leaves and mycelium. Leaves samples were taken at 6, 10 and 12 days after inoculation. The subtracted library was obtained by cloning and transformation of subtracted products and as a result, 600 recombinants clones were obtained. Sequence analysis of sixty nine clones, revealed redundancy of the expressed sequence tags and most of them showed no homology with reported sequences at databases and only 13 % had a high homology with metalothioneins. The results constitute a step in advance in the molecular study of Musa-Mycosphaerella fijiensis interaction.
Authors and Affiliations
Milady Mendoza-Rodríguez, Aminael Sánchez-Rodríguez, Mayra Acosta-Suárez, Berkis Roque, Orelvis Portal, Elio Jiménez
Keywords
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