Construction and partial sequencing from a subtractive library in cut Calcutta 4 ’(Musa AA) in early stage of infection with Mycosphaerella fijiensis Morelet
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 4
Abstract
The study of genes involved in plant defense response against pathogen attack, is one of most important steps leading to the elucidation of disease resistance molecular mechanisms. The generation of subtracted deoxyribonucleic acid libraries (cDNA), by means of suppression subtractive hybridization technique (SSH), has been used for this purpose. A subtractive hybridization was made between a cDNA population obtained from ‘Calcutta 4’ inoculated leaves with M. fijiensis (CCIBP-Pf83) and a mixture of cDNA from ‘Calcutta 4’ non inoculated leaves and mycelium. Leaves samples were taken at 6, 10 and 12 days after inoculation. The subtracted library was obtained by cloning and transformation of subtracted products and as a result, 600 recombinants clones were obtained. Sequence analysis of sixty nine clones, revealed redundancy of the expressed sequence tags and most of them showed no homology with reported sequences at databases and only 13 % had a high homology with metalothioneins. The results constitute a step in advance in the molecular study of Musa-Mycosphaerella fijiensis interaction.
Authors and Affiliations
Milady Mendoza-Rodríguez, Aminael Sánchez-Rodríguez, Mayra Acosta-Suárez, Berkis Roque, Orelvis Portal, Elio Jiménez
Keywords
Related Articles
- EP ID EP684132
- DOI -
- Views 123
- Downloads 0
Relationship between phenotypic characters of plants of ‘Grande naine ’and‘ Calcutta 4 ’grown in vitro and their response to inoculation with Mycosphaerella fijiensis in a greenhouse
Resistance of some varieties of Musa sp. to Black Sigatoka is related to the presence of physical barriers associated with leaf anatomy. However, knowledge about the relationship between morphological characters and th...
An alternative to estimate the retention of atmospheric carbon by Bambusa vulgaris var. Schrad vulgaris. ex J.C. Wendl.
The estimation of atmospheric carbon sequestration by a woody plant has two alternatives for implementation: by identifying the average annual increase in volume or by determining the appropriate method for calculating...
Influence of the genotype and inoculation density on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robust
The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica) and Robusta (Coffea canephora). Cell suspensions with high embryogenic potent...
Establishment and multiplication of cell suspensions of Cymbopogon citratus (D.C) Stapf
Cellular suspensions settled down starting from callus of Cymbopogon citratus (D.C) Stapf cultivated in semisolid medium, according to the methodology described for Freire (1998), for the cultivation of the cane of sugar...
Evaluation of different potato somaclones for resistance to Alternaria solani Sorauer, through the use of artificial inoculation under field conditions
The use of plant pathogens, for the genetic improving programs is an important matter to guarantee its success. Utilization of phytopathogenic isolates from natural source, let us to employ rapid screening in some impor...