In vitro establishment of Phalaenopsis
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 1
Abstract
The present work had as objectives to establish in vitro axillary buds of the floral stalk and to germinate in vitro botanical seeds of Phalaenopsis to obtain the formation of protocorm like bodies. As vegetable material axillary buds of the floral stalk and botanical seeds were used, different disinfection treatments with the employment of the Hypochlorite of sodium like disinfectant agent were carried out. The results showed that it was possible to establish in vitro axillary buds of the floral stalk and to germinate in vitro botanical seeds of this orchid. It was determined in the case of the establishment of the axillary buds that in the treatment with 2.0% of Hypochlorite of sodium and 15 minutes, 20% of the buds died. However, with 1.5% of Hypochlorite of sodium and 15 minutes 80% of sprouted buds was achieved, losses didn’t take place for death and 20% of contamination was presented. It was also observed that like average the axillary buds sprouted to the 13 culture days. With relationship to the disinfection of the botanical seeds, the best treatment turned out to be 1.5% of Hypochlorite of sodium and 20 minutes, this treatment presented 12.5% of contamination. The germination of the botanical seeds began, as average, after 23 days of culture and the first protocorm like bodies were observed after six weeks of culture.
Authors and Affiliations
Manuel de Feria, Maité Chávez, Elisa Quiala
Keywords
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