Regeneration of plants via direct organogenesis in Phaseolus vulgaris L.
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 2
Abstract
An efficient and reproducible procedure for plants regeneration from cells and tissue culture is an indispensable requirement for the application of genes transfer methods in plant breeding. Eventhough, the development of tissue culture protocols in some species of plants, genus Phaseolus and especially Phaseolus vulgaris species have remained recalcitrant to in vitro regeneration. A plant regeneration protocol was need for this species. The influence of two cytokinins in the culture medium to induce multiple buds in cv. CIAP 7247 was tested. Besides, the need to place the explants in a pretreatment with liquid medium before subculturing in buds induction culture medium was also evaluated. Several culture media were studied for plant regeneration starting from these structures. Results showed TDZ is indispensable for multiple buds induction in this variety. Significant differences were observed, only in the number of explants with multiple buds, when 0.25 mg.l -1 TDZ were added in the culture medium. Pretreatment with 1 mg.l -1 TDZ to cigotic embryos germinated in vitro was indispensable to increase percentages of multiple buds formation . The 6-BAP did not influence in plant regeneration.
Authors and Affiliations
Lourdes R. García, Raúl Collado, Idalmis Bermúdez-Caraballoso, Novisel Veitía, Damaris Torres, Carlos Romero
Keywords
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