Optimal concentration of Ethyl Methane Sulfonate for phenotypic variation induction in Phaseolus vulgaris L. cv. ‘DOR 364’
Journal Title: Biotecnología Vegetal - Year 2016, Vol 16, Issue 3
Abstract
Common bean (Phaseolus vulgaris L.) is the main legume used for human consumption worldwide and is an important objective in breeding programs to favor certain characteristics of agronomic interest. In order to determine the optimal concentration of ethyl methane sulfonate to induce phenotypic variation in Phaseolus vulgaris L. cv. ‘DOR 364’ a study was carried out. The seeds were treated with different concentrations of ethyl methane sulfonate (0, 20, 30, 40, 50, 60 mM) and seeded in polyethylene bags containing 4 kg of substrate. Evaluations were carried out on variables involved in germination, growth, foliar development, plant morphology as well as yield components. The ethyl methane sulfonate influenced the different stages of development of the culture that were analyzed. Based on germination and plant development, survival, yield components and frequency of phenotypic variations, 30 mM ethyl methane sulfonate was selected as the optimal concentration to establish a population of possible mutants in common bean cv. ‘DOR 364’. To date, no previous studies have been reported. With the optimum concentration it was possible to generate the greatest number of phenotypic variations in the analyzed population.
Authors and Affiliations
Luis Rojas, Raúl Collado, Aidelyn León García, Leonardo Rivero, Bárbara Ocaña, Marilín Hernández, Novisel Veitía, Amanda Martirena-Ramírez, Dámaris Torres, Lourdes R García
Keywords
Related Articles
- EP ID EP683068
- DOI -
- Views 174
- Downloads 1
Effect of lead on imbibition, germination, and growth of Phaseolus vulgaris L. and Zea mays L.
Lead is highly reactive and it can be consequently toxic to living cells to both plants and humans. This heavy metal is a source of contamination to the environment and it disrupts natural cycles. The present study was...
Filamentous fungi associated with Bambusa plantations vulgaris var. Schrader vulgaris ex Wendland
Bambusa vulgaris var. vulgaris Schrader ex Wendland is a species of a great economic importance and in the conservation of soils. Nevertheless, little information exists on the microbial communities associated to its pl...
In vitro multiplication of ‘FHIA-25’ (Musa spp., AAB) in Systems Temporary Immersion
Necessity to produce high quality planting material has required searching new alternatives to increase the efficiency of in vitro propagation methods and their automation, as in Temporary Immersion Systems (TIS). This...
Effect of carbendazim for the control of Colletotrichum sp., Contaminant of the in vitro establishment of coffee callus
Collectotrichum genus was identified as frequent contaminant in coffee callus formation from foliar explants fragments of plants cultivated in field. The presence of this genus as contaminant in fragments of coffee leave...
In vitro plant acclimatization of Solanum tuberosum (L.) variety Desiree
In the phase of acclimatization of the Plants Biotechnology Institute of the Central University of Las Villas the present work was done with the objective of development the methodology of acclimatization of potato plan...