Use of blue aniline-KOH staining in the study of the banana-Mycosphaerella fijiensis Morelet interaction
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
The analysis of the first stages of the infection process with the fungus Mycosphaerella fijiensis Morelet was made beginning with the inoculation of young banana plants obtained from in vitro culture. The experimental system host- pathogen studied, included the susceptible cultivar ‘Niyarma yik’ (AA) to Black Sigatoka disease and the Pseudocercospora fijiensis isolate CCIBP-Pf1. The fluorescent staining technique with KOH-aniline blue was used for this study. Samples from infected leaves were taken and incubated in a KOH solution, rinsed in deionized water, mounted in the stain solution and examined with ultraviolet fluorescence. The use of this technique allowed to observe the epiphytic growing of the fungus over the plant tissue with high resolution and contrast, in an early stage of the infection.
Authors and Affiliations
Milady F. Mendoza , Elio Jiménez , Frank Maier , Whilhelm Shäfer , Michel Leyva, Mayra Acosta, Yelenys Alvarado
Keywords
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