Histological study of somatic embryogenesis in Agave fourcroydes Lem
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 1
Abstract
A histological study of the structures embryogenic formed from meristem of Agave fourcroydes, with the objective of observing the behavior of the somatic embryogenesis from the induction until the formation of the embryo was carried out. The meristem cultivated in vitro was subculture in modified MS medium the induction. The samples to be included in the resin JB4 were taken periodically. The histologicals cuts of 5-10 µm were carried out with rotatory microtome and strained in 2% toluidine blue. The sections were observed and photographed in light microscopy Axioplan Zeiis with increases of 20X. The results show to the 60 days of culture the presence of cells meristematic pre-embrygenic. Embryos in different phases of formation globular and scutellum were observed in all the mass of the callus in the following subculture.
Authors and Affiliations
Silvia Alemán García, Gerardo González Oramas, Felipe Barredo
Keywords
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