Influence of growth regulators and inhibitors on the multiplication of axillary shoots of the hybrid cultivar 'FHIA-21' (AAAB) in Temporary Immersion System
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 1
Abstract
This work was carried out at the Plant Biotechnology Laboratory from the Instituto de Investigaciones en Viandas Tropicales (INIVIT) in order to determine the culture medium for the multiplication of hybrid ‘FHIA–21’ (AAAB) in Temporary Immersion Systems. Different combinations of growth regulators and growth inhibitors (6-BAP, IAA and PBZ) were studied. Results permitted to determinate the effect of 6-benzil-amino-purine (6-BAP), Indol Acetic Acid (AIA) and Paclobutrazol (PBZ) for multiplication of hybrid ‘FHIA–21’ (AAAB) in the Temporary Immersion System, which involved supplemented MS salts with 2.0 mg.l -1 6-BAP; 0.65 mg.l -1 IAA and 10.0 mg.l -1 ascorbic acid; as well as, 1.0 mg.l -1 paclobutrazol. A decrease of unnecessary growing of shoots and leaves from sprouts in the multiplication phase and a greater sprout number per inoculated explant without multibuds and hyperhydricity were achieved. The Temporary Immersion System allowed to increase the number of axillary sprouts by micropropagation of hybrid ́FHIA-21 ́ (AAAB) and a higher quality of in vitro plants.
Authors and Affiliations
Milagros Basail , Rafael G. Kosky , Victor Medero , Eneida Otero , Marlenys Torres , Manuel Cabrera , Jorge López , Magaly García , Arletys Santos , Aymé Rayas , José de la C. Ventura , Maricel Bauta , Miguel Álvarez , Eriker Páz , Yoel Beovidez , Julia Albert , Alberto Espinosa , Jesús García
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