Influence of the characteristics of plants grown in vitro Bambusa vulgaris var. Schrad vulgaris. ex Wendl. in its acclimatization
Journal Title: Biotecnología Vegetal - Year 2011, Vol 11, Issue 3
Abstract
Acclimatization is a phase of in vitro plants propagation which influences the efficiency of plant regeneration protocols. It is necessary to define the characteristics of a quality plant material to improve acclimatization of Bambusa vulgaris var. vulgaris plants cultured in vitro. The aim of this study was to determine the effect of the characteristics of B. vulgaris var. vulgaris in vitro plants in the acclimatization phase. Then, the influence of the growth development of in vitro plants was considered for their survival during acclimatization. Height and the number of buds and roots per plant were measured after 30 days of planting. Among all the variables, plants height influenced their acclimatization in the greenhouse. Survival rate was significantly different between treatments with plants smaller than 3.0 cm and treatments with plants higher than 3.0 cm. Similar response was obtained after 30 days in the quantification of the number of roots and buds emitted per plant, regardless the presence of roots when planting. Taking into account the results we conclude that the height of the B. vulgaris in vitro plants was the morphological characteristic with major influenced in the survival and development of plants during the acclimatization phase.
Authors and Affiliations
Ortelio Hurtado, Marisol Freire-Seijo, Michel Leiva-Mora, Yudith García-Ramírez
Keywords
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