Callus extracts of Oroxylum indicum (L.) Vent containing baicalein have in vitro antioxidant and antibacterial activities
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 1
Abstract
Oroxylum indicum (L.) Vent is used as a traditional medicine to cure many diseases. The flavonoids baicalein has been detected and extracted from in vitro culture. The aim of this research was to determine the in vitro antioxidant and antibacterial activities of O. indicum callus extracts. Murashige and Skoog medium supplemented with BAP 1 mg l-1 and AgNO3 2 mg l-1 was used to induce the formation of callus. Different explants were excised from 15 days old seedlings developed on MS medium. Metabolites were extracted from two-month-old callus. Ethanolic and aqueous extracts were prepared in cold and hot conditions. They were used for phenolic and flavonoid content determination. Extracts of callus were subjected to thin layer chromatography (TLC), UV –Vis spectrophotometry (range 200-600nm) to check the presence of flavonoid (baicalein) and FTIR. The free radical scavenging activity of the callus extracts were investigated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antibacterial activity was determined by well diffusion method and MTT assay viability test against Micrococcus luteus and Staphylococcus aureus. Dark brown and friable callus of O. indicum was developed. Presence of baicalein was detected by using UV-Visible Spectrophotometer, in purified elute, isolated from TLC of callus extracts. Baicalein was confirmed in extracts of callus on UV–Vis Spectrophotometer against the peak of standard baicalein. Functional groups of baicalein were confirmed by FTIR analysis in the oven dried callus in the range of 400-4000 cm-1. DPPH assay of ethanolic cold extract of callus revealed the best antioxidant property (6.2 ± 0.1 µg ml-1) as compared with the standard (ascorbic acid). Ethanolic extract of callus of O. indicum reveal inhibitory activity against the bacterial strains assayed.
Authors and Affiliations
Rumana Faraz, Mamta Gokhale, Ragini Gothalwal
Keywords
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