Rhizosphere of three cultivars of Phaseolus vulgaris L. source of endospore-forming bacteria with biotechnological potential
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 4
Abstract
Plant Growth Promoting Rhizobacteria constitute a promising source of microorganisms, with the capacity to develop new biopesticides that contribute to pest control, with an agroecological and sustainable basis. The objective of this work was to obtain isolates of endospore-forming bacilli with biotechnological potential from the rhizosphere of different cultivars of bean (Phaseolus vulgaris L.) from three types of soil. Rhizospheric soil collections were made from three bean cultivars present in the municipalities of Colón and Unión de Reyes, in the province of Matanzas, Cuba. The soil samples were grown in Nutrient Broth and the vegetative cells were subsequently removed with heat. Serial dilutions were made and the samples were inoculated in Nutrient Agar culture medium. Bacterial colonies with morphological differences and Gram-positive and endospore-producing bacterial cells were selected. The antagonistic potentialities of the isolates were evaluated through the production of lytic enzymes such as proteases, lipases, cellulases, amylases, chitinases and / or β-glucanases and the synthesis of hydrogen cyanide. A collection of 141 isolates of endospore-forming Gram positive bacilli was made. The 8% of isolates were producers of hydrogen cyanide, 57.7% of proteases, 19.3% of lipases, 56.5% of chitinases and / or β-glucanases, 28.5% of cellulases and 33.3% of α-amylases. These isolates have biotechnological potential with application in the agricultural and industrial sectors.
Authors and Affiliations
Yunel Pérez Hernández, Ana Julia Rondón Castillo, María Caridad Nápoles García, Marlene María Martínez Mora, Yasmary Rubio Fontanills
Keywords
Related Articles
- EP ID EP697692
- DOI -
- Views 42
- Downloads 0
Establishment and multiplication of cell suspensions of Cymbopogon citratus (D.C) Stapf
Cellular suspensions settled down starting from callus of Cymbopogon citratus (D.C) Stapf cultivated in semisolid medium, according to the methodology described for Freire (1998), for the cultivation of the cane of sugar...
Bacterial strains diversity in Musa spp. phyllosphere with antifungal activity against Mycosphaerella fijiensis Morelet
The search for alternatives to agricultural pesticides used for the management of black Sigatoka (Mycosphaerella fijiensis Morelet) includes the selection of microorganisms strains with potential for the control of this...
Determination of genetic stability of in vitro propagated plants of Vasconcellea x helbornii Badillo (Badillo) using ISSR markers
The mountain papayuelo, among them babaco [Vasconcellea x helbornii Badillo (Badillo)], are native of Ecuador and has a great nutritional value, that is it has a huge national and international demand. The objective of...
Multiplication of Ipomoea batatas clone 'INIVITB2-2005' in Temporary Immersion System
Use of temporary immersion system (TIS) have been not reported for in vitro culture of Ipomoea batatas . This technique would help to increase the number of plants needed for in vitro mass propagation of clone ‘INIVITB2...
In silico identification of DELLA proteins in plants of Coffea arabica L. and Ananas comosus L. Merr
Gibberellins control various aspects of plants growing and development, including seed germination, stem elongation, leaves expansion, flower and seed development. They are also involved in phase changes in some species,...