Induction and callus formation of Anthurium andraeanum variety ´Lambada ’ from foliar sections of in vitro plants
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 1
Abstract
Foliar sections of in vitro plants of Anthurium andraeanum variety ‘Lambada’ were used as explants for the induction and formation of callus. The formation of callus was evaluated in two culture mediums (MS and Nitsch, modified) to which the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) were added in four concentrations 2.26, 4.52, 6.78 and 9.04 μM and the addition of 6-Benzilaminopurine (6-BAP) to a concentration of 2.22 μM keeping it constant for both culture mediums. The treatments were placed in darkness at 28±2oC during 45 days. Characteristics of the callus, such as colour and form, were also described . The results showed, in the statistical analysis, a significant interaction among the factors in study (concentrations of the growth regulator and type of culture medium). The calluses obtained presented translucent cream yellow colour with nodular structures. Callus were formed in the two culture medium used, however, the biggest explants percentage that allowed the formation of these structures was observed in the culture medium MS modified when a concentration of 6.78 μM 2,4-D was used . It was demonstrated that it is possible to use foliar sections of in vitro plants to form callus in Anthurium andraeanum variety ‘Lambada’ in the culture medium MS with the nitrates reduced in a half and 2,4-D.
Authors and Affiliations
Nydia del Rivero , Daniel Agramonte , Raúl Barbón , Wilder Camacho, Raúl Collado , Felipe Jiménez-Terry , Marta Pérez, Odalys Gutiérrez
Keywords
Related Articles
- EP ID EP684119
- DOI -
- Views 119
- Downloads 0
Selection of stable and productive sugarcane cultivars at La Estrella sugar company
The evaluation of the sugarcane cultivars response (Saccharum spp.) in different environments is a common practice to select those with the highest production. The objective of the work was to select stable and product...
Cardenolide metabolism and genetic transformation of Digitalis. Potentials and challenges
Cardenolides are secondary metabolites produced by plants of the genus Digitalis. These are widely used in treatments of congestive heart failure. Failed attempts to obtain suitable cardenolide levels from in vitro cul...
Direct somatic embryogenesis in Swietenia macrophylla King
Swietenia macrophylla King is difficult to be propagated by tissue culture and there is not an efficient system via organogenesis, due to problems of microbial contamination, phenolic oxidation and death of tissue in th...
In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa...
Effect of Enerplant® biostimulant onex vitro acclimatization ofin vitropropagated sugarcane plants cv. C97-445
In vitro sugarcane plants (Saccharum spp.) are susceptible to environmental changes in theacclimatization phase, which affects their growth and development. The objective of thiswork was to evaluate the effect of the bio...