Comparison between two methods of establishing embryogenic cell suspensions of banana cv. ‘Grande naine’ (Musa AAA)
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 2
Abstract
Comparison of two methods for the establishment of embryogenic cell suspensions obtained from the culture of male flower directly in liquid culture medium and embryogenic callus of banana cv. ‘Grande naine’ (Musa AAA) was studied in this work. Establishment of homogeneous embriogenic cell suspensions from the culture of both explants in liquid medium was achieved though floral bud range eight showed the best results. Differences in the growing of cell suspension, somatic embryos formation and germination was not observed when the suspensions were compared. Nevertheless the methodology proposed in this work was faster than the ‘callus’ methodology and percentage of establishment was higher.
Authors and Affiliations
Borys Chong, Rafael G. Kosky, Maritza Reyes, Idalmis Bermúdez-Carballoso, Jorge Gallardo-Colina, Marisol Freire-Seijo, Laisyn Posada-Pérez, Idalia Herrera
Keywords
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