In vitro assymbiotic germination of wild orchid seeds
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 3
Abstract
Among Cuban orchid species they are gathered in different threaten categories. There are not reports on how to achieve their mass propagation and in a short term. For these reasons, the study was aimed to achieve in vitro germination of seeds of fifteen species of wild Cuban orchids. This is also a contribution to the rescue and conservation of wild Cuban orchids. Capsules of Bletia patula, Encyclia gravida, Encyclia oxypetala, Encyclia phoenicea, Epidendrum nocturnum, Epidendrum secundum, Epidendrum wrigthii, Eulophia alta, Oncydium luridum, Phaius tancarvillae, Physinga polygonatum, Polystachya concreta, Polystachya foliosa, Prosthechea fragans and Schomburgkia lyonsii were collected, washed and disinfected. Three culture media were used to assess germination of seeds in each species. Results showed, for the first time, the possibilities for in vitro asymbiotic germination of the listed species. Germination of nine species in a period between six and forty weeks was proved. Also the wide variability of responses in this process among the species was observed.
Authors and Affiliations
Loexis Rodríguez, Roberto González, Karen Alvarado, Enidia Telles
Determination of adequate concentrations of 2,4-D and 6-BAP for the induction of morphogenic calli of sweet potato
Tubers from Inivit B 90-1 and Yabú-8, cultivars were put in the laboratories into water bottle and we chose explants of limbs from sprout. Explants were disinfected with Sodium Hypoclorite (Na OCl) with active chlorine (...
In vitro establishment of Zephyranthes spp.
The genus Zephyranthes plants bulbous, herbaceous and perennial, with great ornamental value, because their flowers have a great variety of sizes and colors. The objective of the present work was to in vitro establish of...
Genomic characterization by AFLP of Stevia rebaudiana Bertoni cultivated in the department of Antioquia (Colombia)
Stevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that...
Effects of different types of seed in Agave fourcroydes Lem. variety ‘Sac Ki’ propagation over indicators related to the growth in nursery
A study was conducted during 15 months under nursery conditions, with the objective to evaluate the effect of different types of seed (in vitro grown plants (V), bulbils of in vitro plants (BV), rhizomes of in vitro pla...
Effect of reinvigoration on the in vitro establishment of apices and nodal segments of Cedrela odorata L.
The current research was developed with the objective to decrease the microbial contamination and to increase the survival in vitro of cedar (Cedrela odorata L.). Reinvigoration of mother plants was carried. Sticks of 1...