Phytotoxicity evaluation of an aqueous extract of Padina gymnospora seaweed on Lactuca sativa L. seeds
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 3
Abstract
Padina gymnospora (Kützing) is an algae with pharmacological and biostimulant potentials. The objective of this investigation was to evaluate the phytotoxicity of an aqueous extract of P. gymnospora by means of a bioassay with seeds of Lactuca sativa L. The biomass of P. gymnospora was collected in the eastern zone of the coast of Santiago de Cuba, Cuba. Phytochemical screening of the extract was carried out and the main families of secondary metabolites present in it were determined. The polyphenols were quantified by the Folin- Ciocalteu method. The phytotoxicity bioassay was carried out in vitro, with a duration of 120 hours. Two concentrations of the extract were analyzed: pure extract (P-100) and a 50% dilution (P-50). The negative control was distilled water. The influence of both concentrations on the percentage of relative germination (PGR), the percentage of relative radicle growth (CRR) was evaluated and with these two parameters the germination index (GI) was calculated. In addition, the length of the hypocotyl was measured. A total of six families of secondary metabolites were identified. The most abundant were phenolic compounds and / or tannins and carbohydrates. The concentration of polyphenols was estimated at 0.384 mg g -1 in tannic acid equivalents. The two concentrations of the extract produced more than 95% germination of the seeds. The CRR values were 45.77% for P-100 and 47.15% for P-50, which indicated inhibition of root length. The IG was 46.57% and 45.49% for P-100 and P-50, respectively. The length of the hypocotyl in both treatments exceeded the control. The extract was classified as slightly toxic for the biomodel used.
Authors and Affiliations
Zenia González-Giro , Pedro Luis Batista-Corbal , Yuleidis González-Pérez , Elizabeth Rodríguez-Leblanch , Eber Marcos-Albear
Keywords
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