Evaluation of the effect of the carbendazim-β-cyclodextrin complex for the control of contaminating filamentous fungi of in vitro plant culture
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 2
Abstract
The majority of in vitro contaminant microorganisms affecting plant in vitro culture are bacterias and fungus. These are commonly observed in vivo on plants, but they could cause harmful effect on plant propagated in vitro. The search of new alternatives for their prevention and control is a priority. Carbendazim is the active ingredient of some systemic fungicides, it´s application for fungus contamination on in vitro propagation of plant it has been constrained for their low solubility on water. This problem could be solved by combining this product with a β-ciclodextrina. This paper was focused to determine the effect of carbendazim-β-ciclodextrina versus the contaminant fungi of plant in vitro culture. It was determined the Minimum Inhibitory Concentration (MIC) by the agar dilution method. Thirty four strains were analysed. The carbendazim-β-ciclodextrina complex showed an acceptable antifungal activity versus contaminant. This result could be an useful alternative to control the fungi contamination on plant propagated in vitro.
Authors and Affiliations
Mileidy Cruz-Martín, Mayra Acosta-Suárez, Michel Leiva-Mora, Yelenys Alvarado-Capó, Minerva Lezcano
Keywords
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