Gerbera jamessonii H. Bolus micropropagation
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 3
Abstract
Gerbera is one of the horticultural crops of great demand on the Cuban local market, but its exploitation is limited because with the traditional multiplication methods established, it is not possible to comply with the demands of the seeds. This work was carried out at the biofactory of Las Flores de Servicios Comunales, with the objective of establishing a methodology for the in vitro commercial propagation of Gerbera. Culture media and explant management in the different micropropagation phases were studied. The results obtained showed that with sodium hypochlorite at 0.5% during 10 minutes, a high disinfection of the shoot tips was achieved. With the combination of 6-bencilaminepurine (1.0 mg-l-1) and giberrelic acid (0.1 mg.l-1) in the culture medium, it was possible to increase the number of shoots per explant during establishment. In the multiplication phase, the best results were obtained with a combination of 6-BAP (2.0 mg.l-1) with AIA (0.65 and 1.3 mg.l-1) subculturing the explants greater than 1.0 cm individually in semisolid culture medium. A significant influence on the number of roots and growth of the explants was achieved adding indolacetic acid to the rooting medium and using for the subculture, explants greater than 3.0 cm. The quality of the explants influenced significantly in the acclimatization phase, demonstrating that the plants rooted in vitro should be 3.0 cm in height.
Authors and Affiliations
Pablo Machado Armas, Daniel Agramante, Nelly Almanza Sánches, Drialys Díaz Sánchez, Leinys Gómez Fleites
Keywords
Related Articles
- EP ID EP697813
- DOI -
- Views 68
- Downloads 0
The plant biotechnology: An alternative for the confrontation to the impacts of the climatic change in Cuba?
The expected impacts of the climatic change on the agricultural and forest sectors in Cuba, derived from the evaluations carried out under the First and the Second National Communication of Cuba to the United Nations F...
Use of AFLP markers for molecular characterization of two crops with agricultural interest
The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA),...
In vitro establishment and multiplication of Guettarda clarense, endemic species of Cuba in danger of extinction
The present work was carried out with the objective of achieving the in vitro establishment and multiplication of Guettarda clarense which is an endemic local of Santa Clara city and it is considered in extinction dange...
Effect of the inoculation density in Coffea arabica L. cv. ‘Caturra rojo’ somatic embryos germination in RITA ® Temporary Immersion System
The development of somatic embryogenesis of coffee (Coffea spp.) in liquid culture medium is a viable alternative for the propagation of these species. The use of liquid culture medium and temporary immersion systems c...
Effect of a propolis formulation on the culture media for potato micropropagation var Desirée
In the efforts to improve the quality of the plants in the in vitro culture, different alternatives have been tested and put into practice going from the increasing of the asepsis measures, chemical treatment of the pla...