Influence of the saline formulation in the conservation of sugarcane plants in vitro at two temperatures
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 3
Abstract
The present work had as aim to determine the saline formulation and its ideal concentration, as well as the culture temperature for the conservation of in vitro plants of sugar cane fran the variaty somaclonal IBP 87-100. Different concentrations of the saline formulations were studied MS (25, 50, 75, 100%) and White (75, 100, 125%) associated with two culture temperatures, 18 and 26 ±2ºC. The percentage of survival and the physiological condition of the plants during the period of conservation were decided. The conservation of the plants during six consecutive months was obtained as result when using the formulation MS reduced to 25 and 50%, associated with a culture temperature at 18ºC. This way the growth of the plants was slow and minor the physiological hurts, with values of survival between 58.3 and 69.2%. Whereas the formulation White did not favour the prolongation of conservation period and the plants only could be supported during two months with more than 50% of the foliate area dried. This behaviour could response due to the low levels of nitrogen that contains this formulation.
Authors and Affiliations
Leyanis García-Águila, Juan N. Pérez Ponce, Mayelin Rodríguez, Blanca Pérez, Yudit Martínez, Zoe Sarría
Keywords
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