Bioactivity-Guided fractionations for Anti-Cancer Property of Leaves of Justicia beddomei
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
Aim: The primary objective of the study was to evaluate the possible effects of ethanolic, chloroform extracts and isolated constituents of Justicia beddomei leaves (JB) on against Ehrlich’s ascites carcinoma (EAC) induced cancer on male Swiss albino mice. Tumors were induced by Ehrlich’s ascites carcinoma (EAC) at dose of 200 mg/kg (p.o). Materials and Methods: The EAC was maintained in the ascetic form by the sequential passages in Swiss mice, by means of every 10 days i.p transplantation of 2x106 tumour cells. All the groups except the control were inoculated with 2x106 tumor cells. For tumour inoculation on day 0, the 0.5ml of a 1:10 Ehrlich Ascites Tumour crushed in Hank's balanced salt solution (HBBS) was inoculated by the i.p. route in albino mice. Test compounds were first dissolved in DMSO (10 mM) and then diluted in 0.9% NaCl. Treatment efficiency was assessed in terms of different extracts, and isolated fractions of effects on the tumor volumes of the tumor beard mice relative to the control group. Results: The two parameters of Specific tumor growth delay (SGD) and percentage of life span (ILS %) was calculated. SGD of ethanolic extract, chloroform extract and isolated fractions (JBF2) of Justicia beddomei leaves was -0.83, -0.64 and -0.80 respectively. Similarly, the percentage of life span (ILS %) was 33.33%, 85.71% and 71.42% for ethanolic, chloroform and JBF2 fractions. Conclusion: These finding indicates that the extracts and isolated fractions has significant anti proliferative action against Ehrlich Ascites Tumour- induced carcinoma in animal models. Thus, Justicia beddomei leaves and related products may provide a novel approach to the chemoprevention and treatment of cancer.
Authors and Affiliations
A. M. Krupanidhi, Anand R Hiremath, Akshara Prakash Dabadi, Vinuth Chikkamath, Ekbote D, Dhavan P, Renukaradhya chitti
Keywords
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